Thesis
Pharmacological identification of the 5-HT receptors in the larval midgut of the yellow fever mosquito (Aedes aegypti)
Washington State University
Master of Science (MS), Washington State University
2012
Handle:
https://hdl.handle.net/2376/103777
Abstract
Previous work from our laboratory has shown that serotonin (5-HT) is a major control factor for transepithelial voltage (Vte) and alkalization of the anterior midgut (AMG) and Vte and acidification of the posterior midgut (PMG) of the larval mosquito, Aedes aegypti. Heretofore it has been impossible to associate the effect of serotonin on acid-base transport with particular 5-HT receptor(s) in the larval mosquito gut. Ketanserin (10 µM), a specific antagonist for 5-HT2-like receptors, was effective against 5-HT stimulation of transepithelial voltage (Vte) and alkali secretion by the AMG, but not the Vte and acid secretion by the PMG, whereas Methiothepin (10 [mu]M), which is active against 5-HT1-like and 5-HT7-like, but not 5-HT2-like receptors, was effective against transepithelial voltage (Vte) and alkali secretion in both portions of the midgut. Since 5-HT2 receptors are expected to act through an IP3/DAG pathway to stimulate cytoplasmic Ca2+, this result is in accord with our recent demonstration that a Ca2+ second message is required for initiation of 5-HT stimulated Vte and alkali secretion in the AMG. Cyclic AMP found in both portions of the midgut also conforms with our findings that there is a possibility of a 5-HT1-like or 5-HT7-like or both receptors present.
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Details
- Title
- Pharmacological identification of the 5-HT receptors in the larval midgut of the yellow fever mosquito (Aedes aegypti)
- Creators
- Alphonse Karl Cobbina
- Contributors
- David F. Moffett (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Sciences, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525149001842
- Language
- English
- Resource Type
- Thesis