Thesis
Plasma lipid profile of thoroughbred racehorses before and after supramaximal exercise
Washington State University
Master of Science (MS), Washington State University
2018
Handle:
https://hdl.handle.net/2376/102916
Abstract
Objective: To compare the plasma lipid profile of Thoroughbred racehorses before and after supramaximal treadmill exercise using an untargeted lipidomics approach. Study Design: Prospective pilot study. Animals: Four Thoroughbred horses (1 mare, 1 stallion and 2 geldings). Methods: The maximal oxygen consumption (VO2 max) was calculated for each horse. Horses then underwent treadmill exercise at the predetermined speed for which the oxygen requirement for each horse had been calculated to be 115% VO2 max. Each horse galloped at this speed until fatigue. Venous plasma samples were obtained before (T0) and immediately (T1), 15 (T2) and 30 (T3) minutes post-exercise, and evaluated using an LC/MS untargeted lipidomics approach at the West Coast Metabolomics Center. Data analysis consisted on principal component analysis and 1-way repeated measures ANOVA. Also, the fold change in the signal intensity of the known lipids between T0 and T1, T2 and T3 was calculated to assess for potential biological relevance. Results: 933 lipids were detected. Of these, 130 were "known" as their spectra and retention index are linked to library entries. Supramaximal exercise induced significant changes in the signal intensity of 13 lipids (7 fatty acids and 6 phospholipids). These lipids are ubiquitous in the organism as major components of biological membranes or energy substrates. Additionally, and although not statistically significant, the fold change in the signal intensity of 5 triacylglycerols when comparing T0 with T1, T2 and T3 may be of biological relevance. Conclusions and Clinical Relevance: The findings in this pilot study indicate that supramaximal exercise induces changes in specific plasma lipids in Thoroughbred racehorses. Preliminary data yielded by this pilot study can be used for power analysis and sample size calculation in future lipidomics studies in Thoroughbreds. Future research may clarify the effect of added dietary fat and conditioning in lipid homeostasis in Thoroughbreds; thus, leading to better training and dietary supplementation programs.
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Details
- Title
- Plasma lipid profile of thoroughbred racehorses before and after supramaximal exercise
- Creators
- Lucas Jose Nolazco Sassot
- Contributors
- Macarena G. Sanz (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525282101842
- Language
- English
- Resource Type
- Thesis