Thesis
Plasma lipidomics in healthy foals and in foals experimentally infected with Rhodococcus equi over time: a pilot study
Washington State University
Master of Science (MS), Washington State University
08/2020
DOI:
https://doi.org/10.7273/000000043
Handle:
https://hdl.handle.net/2376/119048
Abstract
Objective: 1) To describe the changes that occur in foal lipidomics as a result of aging (birth to 8
weeks) and 2) To compare these results with those observed after experimental infection with R
equi.
Study Design: Pilot experimental study.
Animals: Thirteen healthy newborn foals with adequate passive transfer.
Methods: Healthy newborn foals (n=9) were challenged with R equi intratracheally the first
week of life. Foals were treated with antibiotics if they developed clinical pneumonia (n=4,
“clinical group”) or were closely monitored if they showed no signs of disease (n=5 “subclinical
group”). An unchallenged group (n=4) was also included. All foals were free of disease
(transtracheal wash fluid evaluation and culture as well as thoracic ultrasonography) by 8 weeks
of life. Plasma lipidomics was determined by LC-MS weekly for the study duration (8 weeks).
Results: Both aging and experimental infection altered the foal’s plasma lipidome as
demonstrated by multivariate statistical analysis. The intensities of 31 lipids was altered by aging and 12 were altered by infection (p<0.05). Furthermore, 9 lipids changed by more than 2-fold
between clinical and subclinical groups.
Conclusions and Clinical Relevance: Aging and R equi infection induced changes in the
plasma lipidome of foals. The results of this pilot study provide the background for future work
in the discovery of earlier biomarkers of R equi pneumonia. Early identification of foals at risk of
developing clinical pneumonia is key in order to decrease antimicrobial use and development of
resistance.
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Details
- Title
- Plasma lipidomics in healthy foals and in foals experimentally infected with Rhodococcus equi over time: a pilot study
- Creators
- Jorge Luis Sanclemente
- Contributors
- MACARENA SANZ (Degree Supervisor) - Washington State University, Veterinary Clinical Sciences, Department ofKELLY D FARNSWORTH (Committee Member) - Washington State University, UNKNOWNDEBRA C SELLON (Committee Member) - Washington State University, Veterinary Clinical Sciences, Department of
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Format
- pdf
- Number of pages
- 71
- Identifiers
- 99900590963701842
- Language
- English
- Resource Type
- Thesis