Thesis
Prediction of isotachophoresis steady-state zone position and analysis of zone behavior around a 180 turn
Washington State University
Master of Science (MS), Washington State University
2007
Handle:
https://hdl.handle.net/2376/102471
Abstract
Isotachophoresis (ITP) has the ability to both concentrate and purify analytes, and thus is a candidate for the purification of low abundance species found in many systems, including human blood serum. In this thesis, the theory and background of ITP is presented followed by two novel articles prepared for submission to scientific journals. The first article develops an analytical expression to predict the location of stationary steady-state ITP zones. Theoretical zone position predictions were compared to experimental results obtained using a vortex stabilized electrophoresis apparatus. The analytical model was found to predict all experimental zone positions within the 95% confidence interval of the data. The second article presents a 2-D simulation of ITP in two turn, or S channel. The introduction of turns into a channel provides two sources of dispersion, both from the difference in electric field strength, and distance a zone must travel across the width of the channel. The simulation presented shows that ITP zones are able to recover from the dispersion introduced by the turns in a straight segment following the turn. The effects of turn radius, voltage and sample mass on the length of straight segment required for zone recovery are presented.
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Details
- Title
- Prediction of isotachophoresis steady-state zone position and analysis of zone behavior around a 180 turn
- Creators
- Schurie Lanette Moreau Harrison
- Contributors
- Cornelius F. Ivory (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525072101842
- Language
- English
- Resource Type
- Thesis