Thesis
Protective effects of dietary goji berry supplement on experimental model of inflammatory bowel disease
Washington State University
Master of Science (MS), Washington State University
05/2016
Handle:
https://hdl.handle.net/2376/102172
Abstract
Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease, (CD) are gastrointestinal disorders characterized by intestinal inflammation. Goji berry (Lycium barbarum) is a traditional Chinese herbal medicine that has long been recognized for the health beneficial effects such as anti-oxidative effects and diabetes prevention. Nonetheless, little is known regarding its effects on dextran sulfate sodium (DSS)-induced colitis, a common colitis mice model for IBD research. In current study, C57BL/6J male mice were first fed a standard rodent diet with or without 1% (w/w) goji berry for 4 weeks, then colitis was induced by supplementing 3% DSS in drinking water for 7 days, followed by a 7-day recovery period to mimic symptoms of IBD. As a result, goji berry supplementation had no effects on body weight gain and feed intake in mice without DSS administration. In DSS induced groups, goji berry ameliorated body weight loss, diminished diarrhea and gross bleeding, resulting in a significantly decreased disease activity index associated with diminished DSS induced colon shortening. Histologically, goji berry supplementation improved colonic edema, mucosal damage in DSS induced mice. Furthermore, neutrophil infiltration into intestinal tissue was prevented by goji berry supplementation. In addition, goji berry suppressed expression of the pro-inflammatory marker interlukin-6 (IL-6), cyclooxygenase-2 (COX-2), and chemoattractants chemokine (C-X-C motif) ligand-1 (CXCL-1) and monocyte chemoattractant protein-1 (MCP-1) in colon tissue induced by DSS. Moreover, no mortality was observed in goji berry diet group. In conclusion, this study demonstrates that dietary goji berry supplementation confers protective effects against DSS induced colitis, suggesting a potential therapeutic value for IBD patients.
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Details
- Title
- Protective effects of dietary goji berry supplement on experimental model of inflammatory bowel disease
- Creators
- Yifei Kang
- Contributors
- Meijun Zhu (Chair)Barbara Rasco (Committee Member) - Washington State University, Food Science, School ofCharles Gould Edwards (Committee Member) - Washington State University, Food Science, School ofMin Du (Committee Member) - Washington State University, Animal Sciences, Department of
- Awarding Institution
- Washington State University
- Academic Unit
- Food Science, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Number of pages
- 105
- Identifiers
- 99900525081401842
- Language
- English
- Resource Type
- Thesis