Thesis
QTL analysis of end-use quality and stripe rust resistance traits in a double haploid soft white wheat population
Washington State University
Master of Science (MS), Washington State University
05/2020
DOI:
https://doi.org/10.7273/000004107
Handle:
https://hdl.handle.net/2376/124970
Abstract
Wheat (Triticum spp.) is a crop of major economic and dietary importance. In the Pacific Northwest region of the United States, soft white wheat is the primary class of wheat grown and is an important export product for the region. Soft white wheat flour is especially important in producing products such as cookies, crackers, pancakes, noodles and pastry products. The goal of this project was to map quantitative trait loci (QTL) for end-use quality and stripe rust (Puccinia striiformis f. sp. tritici) resistance in a double haploid mapping population derived from a cross between a soft white winter club wheat, 'Cara', and a soft white winter lax wheat, 'Xerpha'. The mapping population of 126 individuals was grown across 12 environments from 2013 to 2017 and rated for stripe rust resistance. In a separate experiment, it was grown in 16 different locations from 2013 to 2016 and phenotyped for 16 end-use quality traits. A linkage map of 23 linkage groups was constructed for the population using 1282 genotyping-by-sequencing (GBS) and KASP markers. QTL analysis revealed 35 significant QTL for stripe rust resistance. Two of the most interesting markers in the stripe rust resistance analysis were IWB38687 and A2927, which were each associated with three separate QTL. Analysis of the end-use quality trait data identified 22 significant QTL. Some of the most significant QTL identified for end-use quality traits had LOD scores well over five and explained over 15% of the variance in the trait they were associated with. Some of the markers identified in this study could be good targets for marker assisted selection in PNW wheat breeding programs, especially because the parents of our mapping population are commonly used in breeding programs in the PNW.
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Details
- Title
- QTL analysis of end-use quality and stripe rust resistance traits in a double haploid soft white wheat population
- Creators
- Carmen Johanne Barkley
- Contributors
- Kimberly Campbell (Advisor) - Washington State University, Department of Crop and Soil Sciences
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Crop and Soil Sciences
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900890785901842
- Language
- English
- Resource Type
- Thesis