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Embargoed Access, Embargo ends: 10/07/2027
Thesis
ROLE OF METHIONINE TRANSPORTER FUNCTION IN SULFUR PARTITIONING AND SOURCE-SINK PHYSIOLOGY OF NODULATED PEA (Pisum sativum L.) PLANTS
Master of Science (MS), Washington State University
2025
Abstract
Sulfur (S) is an essential nutrient that plants require for the synthesis of methionine and cysteine. These S-amino acids are building blocks of enzymes and proteins and are needed to produce other S-containing compounds crucial for plant growth, development, and stress response. In legumes like peas (Pisum sativum L.), S is particularly important for the process of atmospheric nitrogen (N) fixation within root nodules, and for the synthesis of high-quality, S-rich storage seed proteins. In this study, it was hypothesized that S partitioning processes from source organs, such as roots and mature leaves, to nodule and seed sinks impact their physiological function. It was further predicted that transporters located within source and sink organs control the quantity and quality of S compounds being transported to nodules or seeds and thereby influence N fixation and nodule function as well as seed metabolism and nutritional value. As S, N and carbon (C) transport and metabolic pathways are linked, it was also anticipated that changes in S partitioning processes would affect N and C metabolism and partitioning, together impacting plant growth and productivity. This project analyzed nodulated transgenic pea plants expressing the yeast Methionine UPtake 1 (MUP1) transporter to better understand the role of S source-to-sink partitioning processes in biological N fixation and plant growth, and in seed yield and protein quality.
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Details
- Title
- ROLE OF METHIONINE TRANSPORTER FUNCTION IN SULFUR PARTITIONING AND SOURCE-SINK PHYSIOLOGY OF NODULATED PEA (Pisum sativum L.) PLANTS
- Creators
- Elizabeth A Campbell
- Contributors
- Mechthild Tegeder (Advisor)Hanjo Hellmann (Committee Member)Rebecca McGee (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- School of Biological Sciences
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Number of pages
- 117
- Identifiers
- 99901296995501842
- Language
- English
- Resource Type
- Thesis