Thesis
Rapid toxicity assessment using Colpoda steinii: toxicity of ibuprofen, naproxen, and acetaminophen
Washington State University
Master of Science (MS), Washington State University
2017
Handle:
https://hdl.handle.net/2376/100550
Abstract
Pharmaceuticals and Personal Care Products (PPCP's) are increasingly found in many water systems around the world. These include over-the-counter pain medications, prescribed medications like antibiotics and antidepressants, and care products such as detergents and fragrances. A rapid microscale bioassay using the soil ciliate Colpoda steinii was developed to determine growth inhibition in the presence of three common over-the counter pain relievers including ibuprofen (IBP), naproxen (NAP), and acetaminophen (APAP). The formulated over-the-counter product solutions, along with the pure active pharmaceutical ingredient (>98%) found in each compound, were analyzed in tandem. Experiments were conducted to estimate the sensitivity of ciliate population growth in the presence of each toxicant solution. Experiments consisted of four replications of 4-5 test concentrations plus controls. Toxicants were introduced to experiment cultures using a log base 10 scale and population growth was observed after a 48- hour period. Standard dose responses based on preliminary tests ranged from 10mgL-1 to 1800 mgL-1 depending on the toxicant. Simple regression and one-way ANOVA statistical methods were used to identify the EC20, EC50, NOEC, and LOECof each toxicant. Use of C. steinii for this type of bioassay addressing PPCP's in water systems, is fitting because it is rapid, cost-effective, reliable, and can represent similar ciliate species in understanding microbial community dynamics.
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Details
- Title
- Rapid toxicity assessment using Colpoda steinii
- Creators
- Geoffrey Donald Schramm
- Contributors
- James R. Pratt (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Environment, School of the (CAHNRS)
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525288201842
- Language
- English
- Resource Type
- Thesis