Thesis
Rapid triacylglyceride detection in live oleaginous micro-organisms with quantification in phototrophic microalgal cultures via liquid state ¹H NMR
Washington State University
Master of Science (MS), Washington State University
2012
Handle:
https://hdl.handle.net/2376/100753
Abstract
Non-invasive methods for measuring lipid content in live oleaginous microorganism cultures are critically needed for biofuel research and development. A nondestructive method requiring minimal sample preparation was developed utilizing liquid state ¹H NMR for quantifying triacylglycerides (TAGs) in live algae cultures. ¹H NMR and ¹H HR-MAS spectra of live algae cells show outstanding correlation with published chemical shifts for TAGs. ¹H NMR spectra of Chlamydomonas reinhardtii whole cells and isolated lipid bodies were compared with a standard oleic TAG ¹H spectrum, demonstrating that only lipid body TAG’s were observed. A “model” TAG was derived, providing a proton count and molecular weight for conversion of TAG ¹H NMR integrals to volumetric TAG or fatty acid methyl ester (FAME) equivalent concentrations, which were correlated to FAME by gas chromatography (FAME-GC) at several time points. A customized NMR flow cell was subsequently constructed, allowing real-time, continuous measurements of vii multiple cultures. TAGs were able to be detected in heterotrophically grown microalgae and yeast by both ¹H NMR and ¹H HR-MAS.
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Details
- Title
- Rapid triacylglyceride detection in live oleaginous micro-organisms with quantification in phototrophic microalgal cultures via liquid state ¹H NMR
- Creators
- Peter Davey
- Contributors
- Shulin Chen (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Biological Systems Engineering, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525109701842
- Language
- English
- Resource Type
- Thesis