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Safety and immunogenicity of a Mycolplasma ovipneumoniae bacterin in domestic sheep
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Safety and immunogenicity of a Mycolplasma ovipneumoniae bacterin in domestic sheep

Jessie Carol Ziegler
Washington State University
Master of Science (MS), Washington State University
2013
Handle:
https://hdl.handle.net/2376/100212
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Abstract

Epizootic pneumonia is a major population limiting factor in the re-establishment of bighorn sheep in western North America following their near-extirpation in the early 20th century. Mycoplasma ovipneumoniae, an opportunistic pathogen frequently carried by domestic sheep and goats, is a primary epizootic agent of epizootic pneumonia of bighorn sheep. Our long term objective is to develop interventions that reduce M. ovipneumoniae exposure of bighorn sheep. The objective of this preliminary study was to evaluate the safety and immunogenicity of M. ovipneumoniae immunization of domestic sheep in 3 separate experiments including: 1) live M. ovipneumoniae (50 ug protein); 2) killed M. ovipneumoniae (50 ug whole cell protein) in oil adjuvant; and 3) M. ovipneumoniae (250 ug whole cell protein) in oil adjuvant. All immunizations were administered twice at 28 day intervals. Circulating antibody was monitored by competitive Enzyme-linked immunosorbent assay (cELISA) and by serum inhibition. Experiment 3 ewes lambed between days 50 and 60 after initial immunization and blood samples were obtained from lambs between 24 hrs and 7 days of age to monitor passive antibody acquisition by cELISA and serum inhibition. Experiment 1 ewes showed no adverse reactions locally or systemically but no evidence of specific antibody responses. Experiment 2 and 3 ewes showed local inflammation and dermal fibrosis at the immunization site, and Experiment 3 ewes had febrile and transient fibrinogen responses to the day 28 booster immunization. Experiment 2 ewes showed no evidence of specific antibody responses but Experiment 3 ewes and their lambs had strong cELISA serum antibody responses and significant serum M. ovipneumoniae inhibition activity. The results of these trials indicated that immunization with relatively large antigen mass in oil adjuvant was required for the development of strong active antibody responses in ewes and passive antibody in lambs.;Immunogenetics.;Sheep--Immunology.;Goats--Immunology.;Goats--Diseases.;Sheep--Diseases.;Immunization.

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