Thesis
Selection of Camelina mutants resistant to acetolactate sythase inhibitor herbicides
Washington State University
Master of Science (MS), Washington State University
2010
Handle:
https://hdl.handle.net/2376/101326
Abstract
Camelina (Camelina satvia L.) is a new alternative crop being developed as a low input, oilseed crop in the Inland Pacific Northwest. Adoption has been slow, in part because of limited weed control options and sensitivity to residual herbicide activity in soils. Other crops with resistance to these herbicides have been developed through mutation. Development of camelina with this resistance would allow great flexibility for the crop in rotation with other crops. M2 seed from camelina mutagenized by exposure to 0.3% ethyl methane sulfonate (EMS) was screened for increased resistance with imazethapyr and sulfosulfuron. Five lines with resistance were identified and characterized. Four mutants identified in a screen for imazethapyr resistance (IM1, IM6, IM10, and IM18) appeared phenotypically identical and are likely controlled by the same semi-dominate gene. One mutant, designated SM4, identified in a screen for sulfosulfuron resistance was phenotypically different and was also controlled by a semi dominant gene. Plants treated with imazethapyr, sulfosulfuron, and flucarbazone were evaluated visually and for biomass production. All mutants appeared more resistant than the wild type visually, and SM4 appeared significantly more resistant than the IM mutants. The imazethapyr dose required to reduce plant growth 50% (GR50) for Calena, IM1, and SM4 was 0.99mg ai/ha, 19.21mg ai/ha and 204.15mg ai/ha, respectively. GR50 values for sulfosulfuron were 0.10 mg ai/ha, 0.46mg ai/ha and 15.56mg ai/ha, and for flucarbazone 1.5mg ai/ha, 4.67mg ai/ha and 10.57mg ai/ha. The herbicide concentration required to reduce enzyme activity by 50% (AR50) was calculated by a crude enzyme extract containing ALS from Calena, IM1, and SM4. AR50 concentrations of imazethapyr for Calena, IM1, and SM4 were 86.29uM, >400uM, and >400uM, respectively. AR50 concentrations of sulfosulfuron were 0.0061uM, 4.04uM, and 13.81uM and of flucarbazone were 0.62uM, 1.72uM, and 1.36uM respectively. Both IM1 and SM4 mutants were confirmed to have resistance to imazethapyr, sulfosulfuron, and flucarbazone. These mutant lines are available to industry and public breeders to create camelina varieties with increased resistance to these ALS herbicides.
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Details
- Title
- Selection of Camelina mutants resistant to acetolactate sythase inhibitor herbicides
- Creators
- Dustin Timothy Walsh
- Contributors
- Scot H. Hulbert (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Crop and Soil Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525165701842
- Language
- English
- Resource Type
- Thesis