Thesis
Sequence and tissue expression of the ABCB4 (MDR3) gene in the canine
Master of Science (MS), Washington State University
2008
Handle:
https://hdl.handle.net/2376/101830
Abstract
The ABCB gene subfamily of ABC transporters transports ligands across biological membranes. ABCB4 functions primarily on the apical membrane of hepatocytes to translocate lipids. ABCB4 translocates the phospholipid phosphatidylcholine from the inner to the outer leaflet of the canalicular membrane in people. Functional alterations in the ABCB4 transporter have been associated with a number of cholestatic diseases in people. Furthermore, Abcb4 (-,-) knockout mice have also been demonstrated to develop hepatic and biliary injury. Approximately 97% of the canine ABCB4 was sequenced and its tissue distribution was determined. There was approximately 99% similarity and identity between ABCB4 consensus sequence reported here and the reported canine sequence with most of the variability occurring within exons 26 and 27. The sequenced ABCB4 gene shared a splicing variant with human ABCB4 variant A. Canine ABCB4 was abundantly expressed in the liver, sparsely expressed in the brain, and not expressed in the duodenum. The plentiful expression of ABCB4 in canine hepatic tissue supports a potential role for the protein in normal biliary function similar to that in people. Tissue distribution, along with characterization, of the ABCB4 gene in the canine will provide the foundation for future investigation into the physiologic function of ABCB4 in the dog. As with people, it is likely that mutations in ABCB4 are responsible for, or associated with, cholestatic disease in dogs.
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Details
- Title
- Sequence and tissue expression of the ABCB4 (MDR3) gene in the canine
- Creators
- Erick Spencer
- Contributors
- Katrina L. Mealey (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Identifiers
- 99900525287501842
- Language
- English
- Resource Type
- Thesis