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Thesis
TITER AND DISTRIBUTION OF HOP STUNT VIROID AND HOP LATENT VIROID INFECTING HOPS
Washington State University
Master of Science (MS), Washington State University
01/2021
DOI:
https://doi.org/10.7273/000005477
Handle:
https://hdl.handle.net/2376/125053
Abstract
Hop stunt viroid and Hop latent viroid have proven recalcitrant to attempts to eliminatethem. This project was undertaken to understand the localization of the two viroids within the
hop cultivars, ‘Comet,’ ‘Chinook,’ ‘Canadian Red,’ and ‘Wuerttemberger’ under cold and dark
stress with the goal of improving viroid elimination therapies. With micro-shoot tip culture,
HSVd elimination was successful in 4% to 9% of regenerated meristems and HLVd elimination
was successful 19% to 54% in ‘Comet’ and ‘Chinook,’ respectively. HLVd was not eliminated in
‘Canadian Red’ and ‘Wuerttemberger.’ In situ hybridization showed that HSVd was present in
the ground meristem, but not the tunica, while HLVd was not present in the first ~500 μM in
‘Comet’ and ‘Chinook.’ HLVd was present in the ground meristem of ‘Wuerttemberger.’ This
localization may explain the difficulty of eradicating the viroids from infected hops. Dark
therapy increased viroid titers and is not advisable. Though cold therapy decreased viroid titers,
meristems failed to regenerate. Unexpectedly, sourcing shoots from plants during spring that
experienced a natural dormancy period showed an increase in HLVd elimination (ranging from
50% to 100%) in cvs. ‘Cascade,’ ‘Centennial,’ ‘Triumph,’ and ‘Wuerttemberger.’ This increase in
HLVd elimination, accompanied by a 13-fold increase in HSVd elimination, was observed in
‘Comet’ plants that experienced an artificially induced spring.
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Details
- Title
- TITER AND DISTRIBUTION OF HOP STUNT VIROID AND HOP LATENT VIROID INFECTING HOPS
- Creators
- Ninh Lien Khuu
- Contributors
- Scott J Harper (Advisor)Michael Knoblauch (Committee Member)Hanu Pappu (Committee Member)
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Plant Pathology
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Number of pages
- 132
- Identifiers
- 99900591955401842
- Language
- English
- Resource Type
- Thesis