Thesis
Taenia Taeniaeformis: differential staining on onchospheres with vital dyes under critical temperatures
Washington State University
Master of Science (MS), Washington State University
2006
Handle:
https://hdl.handle.net/2376/102887
Abstract
Ingestion of T.saginata eggs by cattle causes a condition called bovine cysticercosis (BC). Hence, control treatments that inactivate T. saginata eggs would be valuable, which requires accurate in vitro methods for assessment of egg viability. Taenia taeniaeformis, a rodent cestode was used as a model and the affect of 0.5% NaOCl on onchosphere recovery from both untreated and heat treated eggs was investigated. Further, the use of non-vital and vital dyes to assess the egg/onchosphere viability under different heat treatments was also studied. Onchosphere recovery of T. taeniaeformis eggs by exshelling with 0.5% NaOCl for 5, 10 or 20 minutes was assessed that showed quadratic loss in recovered eggs and onchospheres with increased incubation time (p<0.05). Also, the effect of heat on the onchosphere recovery was tested by exposing eggs to 55, 65 or 75° C for 10 minutes. Heat treatment at 75° C resulted in remarkable onchosphere loss when exshelled by 0.5% NaOCl (p<0.05). Non-vital dye acridine orange (AO) or vital dye propidium iodide (PI) stained eggs either peripherally or internally, while vital dye trypan blue (TB) showed only peripheral staining in eggs, but showed variable staining in NaOCl exshelled onchospheres. Preliminary studies at low temperatures indicated differences in the staining behavior of the vital dyes, but not between AO and PI. Experiments at higher temperature (55-95° C) were performed for better resolution of the staining behavior. AO or PI was used directly with heat treated eggs, while TB was used with onchospheres that were exshelled prior to heat treatment. This is because of the onchosphere loss mentioned earlier. Non-linear regression analysis on the obtained percentage internal staining data with each dye by fitting four-parametric sigmoid model showed that T50 for AO was lower that that of PI, which in turn was lower than that of TB. From these findings, the use of vital dyes appears to be an unacceptable means to assess viability of onchospheres within eggs or onchospheral membranes.
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Details
- Title
- Taenia Taeniaeformis
- Creators
- Kalyan C. Chapalamadugu
- Contributors
- Jan Roger Busboom (Degree Supervisor)Mark Loge Nelson (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Animal Sciences, Department of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525171601842
- Language
- English
- Resource Type
- Thesis