Thesis
The effect of modified ADP-glucose pyrophosphorylase activity on starch metabolism In planta
Washington State University
Master of Science (MS), Washington State University
2008
Handle:
https://hdl.handle.net/2376/103200
Abstract
In rice, long-term starch storage accounts for 75-80% of the seed dry weight. Upregulated transgenic rice containing Escherichia coli glgC-triple mutant gene which encodes a bacterial AGPase led to three to six-fold increase in AGPase activity and 7- 24% enhanced seed biomass compared to wildtype Kitaake. In order to obtain additional understanding of these transformants, metabolite analysis of up-regulated transgenic lines along with wildtype was performed. Increased levels of ADP-glucose, a product of AGPase, and hexose pools such as glucose 1-phosphate and glucose 6-phosphate, were observed in all up-regulated transgenic rice. The change in hexose-phosphate pools indicates that some other factor besides AGPase limits starch biosynthesis in rice endosperm. Transport of ADP-glucose into amyloplasts and/or restricted activities of starch biosynthesis enzymes are the likely processes which may be limiting rice starch production in these transgenic plants.
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Details
- Title
- The effect of modified ADP-glucose pyrophosphorylase activity on starch metabolism In planta
- Creators
- Yasuko Nagai
- Contributors
- Thomas W. Okita (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Program in Molecular Plant Sciences
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525130701842
- Language
- English
- Resource Type
- Thesis