Thesis
The effects of additive solutions on the development of storage lesions in canine platelet concentrates stores at 4°C
Washington State University
Master of Science (MS), Washington State University
08/2020
DOI:
https://doi.org/10.7273/000004142
Handle:
https://hdl.handle.net/2376/124889
Abstract
Canine platelet transfusions are an essential tool to treat acute bleeding or prevent surgical complications in dogs with platelet disorders. Currently long-term storage is not advised, limiting the options for a potentially life-saving treatment, especially in emergency situations. The goal of this study was to demonstrate that platelet additive solutions (PAS) can be used in place of plasma and that these solutions, when combined with cold storage at 4⁰C, reduce the occurrence and degree of platelet storage lesions, maintain platelet function, delay pathogen proliferation, and extend storage time of platelet concentrate for up to 7 days. Platelet concentrates obtained from canine blood donors, were aliquoted into 4 separate bags containing 100% plasma (control) or 35% plasma and 65% of a PAS (Plasma-Lyte A, Isoplate, or InterSol). Samples were stored at 4⁰C without agitation. At days 0, 3, 5, and 7 samples were analyzed to determine the degree of platelet storage lesion as evaluated by measurement of metabolic markers, platelet activation markers, platelet aggregometry assessment; and bacterial growth. Platelet count and mean platelet volume, glucose, lactate, lactate dehydrogenase, pO2, pCO2, aggregation percentage via light aggregometry, activation percentages via flow cytometry detection of surface P-selectin, and bacterial contamination via culture were assessed to compare the 4 storage solutions. Development of storage lesions was minimal, as demonstrated by maintenance of a mean pH >7.2 (p < 0.05) and mean lactate values < 6 mmol/L at day 7 in all solutions (p < 0.05). There were differences in the activation percentage between the units, but was overall low in all the solutions. Glucose utilization did not vary significantly between any of the solutions. No significant difference was found between plasma and platelet additive solutions for PO2 and PCO2. No bacterial growth was found in any of the solutions. Overall the PAS were comparable to plasma for the cold storage of platelets. Cold stored platelets showed minimal storage lesion development with no bacterial growth. Plasma, Plasma-lyte A and Isoplate stored platelets maintained function for up to 7 days at 4⁰C.
Metrics
2 File views/ downloads
27 Record Views
Details
- Title
- The effects of additive solutions on the development of storage lesions in canine platelet concentrates stores at 4°C
- Creators
- Sara Ravicini
- Contributors
- Jillian Marie Haines (Advisor) - Washington State University, Veterinary Clinical Sciences, Department of
- Awarding Institution
- Washington State University
- Academic Unit
- Veterinary Medicine, College of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900890779901842
- Language
- English
- Resource Type
- Thesis