Thesis
The effects of strontium-doped sol-gel and plasma-sprayed coatings on osteoblasts in static and dynamic culture conditions
Washington State University
Master of Science (MS), Washington State University
2017
Handle:
https://hdl.handle.net/2376/102702
Abstract
An aging population, increased life expectancy and quality of life, and a rise in the number of younger, active patients requiring a metal implant has magnified the need for longer lasting implants. In order to test new materials, researchers must rely on static cell cultures or animal models, each have drawbacks to studying and confirming the efficacy of new implant materials. It is therefore the objective of this research are to modify the surface of Ti-6Al-4V ELI disks to enhance the bioactivity for implant applications in orthopedics and dentistry while improving the adhesion strength of bioceramic coatings. Additionally, it was the goal of this research to design and build a perfusion flow bioreactor, and demonstrate its use with an in vitro cell culture for use with future samples and cell lines. The hypothesis of the first aim is that the combination of TiNT with a sol-gel method of coating will increase the adhesion strength while improving bioactivity through the addition of strontium dopant. The hypothesis of the second aim is that the bioreactor will be able to accommodate different sample designs and culture cells as effectively as static cultures. In this study, titania nanotubes (Ti-NT) were grown on the surface of Ti64 ELI disks via anodic deposition, heat treated, and coated with pure hydroxyapatite (HA) and strontium-doped hydroxyapatite (Sr-HA) sol-gel coatings. In vivo results showed no significant difference between both plasma sprayed HA and Sr-HA and sol-gel coated HA and Sr-HA. Adhesion strength testing of the sol-gel coated samples showed that the HA coatings had strengths of 20.64 ± 2.37 MPa while Sr-HA exhibited 24.49 ± 10.99 MPa. The perfusion flow bioreactor was able to successfully culture osteoblast cells, with no significant difference between the reactor and static cell culture MTT data.
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Details
- Title
- The effects of strontium-doped sol-gel and plasma-sprayed coatings on osteoblasts in static and dynamic culture conditions
- Creators
- Samuel Ford Robertson
- Contributors
- Susmita Bose (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Mechanical and Materials Engineering, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; [Pullman, Washington] :
- Identifiers
- 99900525057101842
- Language
- English
- Resource Type
- Thesis