Thesis
The identification of some unknown Allium accessions for ornamental value
Washington State University
Master of Science (MS), Washington State University
12/2019
DOI:
https://doi.org/10.7273/000003972
Handle:
https://hdl.handle.net/2376/123078
Abstract
Allium have a long history of use. Leeks, onions, garlic are some of members of Allium, and Alliums are also used as ornamental plants. This study focused on (1) the phenotypic evaluation of ornamental Alliums and (2) the identification of unknown Alliums spp., with potential ornamental value, using DNA barcoding. The Alliums studied were from the USDA collection held by the Western Regional Plant Introduction Station. For evaluation of ornamental Alliums, the morphological features of 14 ornamental Allium accessions were measured at flowering in the field. To be considered for use as a cut flower, an accession's scape should be longer than 60 cm. Twelve of 14 ornamental Alliums measured in the field met this criterion. The umbel diameter ranged from 0.3 cm to 14.4 cm, and six different flower colors were observed: purple, yellow, cream, mix, blue and white. Species determination was proposed for 16 of unknown Allium accessions using the DNA barcode gene ITS. Five of these accessions with good ornamental characteristics, ITS regions were genetically identified to species A. cretaceum (W6 26738, W6 23154), A. elegans (W6 23371), A. senescens (W6 1435), A. atroviolaceum (W6 17165). These accessions should be further studied morphologically to confirm these species identifications. ITS sequence will be useful for the preliminary identification of unknown Allium species in plant genetic resource collections.
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Details
- Title
- The identification of some unknown Allium accessions for ornamental value
- Creators
- Aysegul Eroglu
- Contributors
- Clarice J Coyne (Advisor) - Washington State University, Department of Crop and Soil Sciences
- Awarding Institution
- Washington State University
- Academic Unit
- Department of Crop and Soil Sciences
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900890803601842
- Language
- English
- Resource Type
- Thesis