Thesis
The use and limitations of the 16S rRNA sequence for species classification of Anaplasma samples
Washington State University
Master of Science (MS), Washington State University
05/2020
DOI:
https://doi.org/10.7273/000004063
Handle:
https://hdl.handle.net/2376/124985
Abstract
Expanded availability of high-throughput sequencing has provided new lines of genetic evidence that have resulted in almost twenty new species proposals for the genus Anaplasma since the last formal taxonomic organization of the genus. This thesis evaluates the potential new species of Anaplasma utilizing 16S rRNA sequences from across the Anaplasma genus and provides guidance for further sequencing and data collection. It demonstrates that 16S rRNA sequences must be used with caution when attempting to define species within the genus Anaplasma, and furthermore provides analysis of the available 16S rRNA sequences to determine key bases that allow a sample classification of the traditionally recognized species of Anaplasma.
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Details
- Title
- The use and limitations of the 16S rRNA sequence for species classification of Anaplasma samples
- Creators
- Mitchell Tyler Caudill
- Contributors
- Kelly A Brayton (Advisor) - Washington State University, Department of Veterinary Microbiology and Pathology
- Awarding Institution
- Washington State University
- Academic Unit
- College of Veterinary Medicine
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University
- Identifiers
- 99900890791301842
- Language
- English
- Resource Type
- Thesis