Thesis
Validation of a 2% lactic acid antimicrobial rinse as an alternative to chlorine for mobile poultry slaughter operations
Washington State University
Master of Science (MS), Washington State University
2009
Handle:
https://hdl.handle.net/2376/102284
Abstract
Poultry is a known source of foodborne pathogens; therefore, processing interventions to reduce pathogens are critical. Washington mobile poultry slaughter operators wanted to identify an antimicrobial rinse alternative to chlorine to assist with product organic labeling and increase consumer appeal. Lab and field studies were performed evaluating lactic acid as an antimicrobial rinse for poultry. The lab study examined water, chlorine (50-100ppm) and lactic acid (2%) as antimicrobial interventions to reduce Salmonella spp. on inoculated chicken wings. Three replications were performed. For each replication 20 inoculated wings were subjected to each treatment (no rinse, water, chlorine and lactic acid rinse) and were examined for Salmonella on Xylose Lysine Desoxycholate (XLD) agar. The Salmonella counts for lactic acid rinsed wings (0.39 log10 cfu/wing) were significantly (p< 0 0.01) lower as compared to water rinsed (5.81 log10 cfu/wing) and chorine rinsed wings (5.69 log10 cfu/wing). For the field study, effectiveness of lactic acid and chlorine rinses in mobile poultry slaughter operations was examined along with incidence of Salmonella. Two replications were performed with twenty carcasses randomly selected per treatment for each replication. Whole carcasses were sampled either immediately after evisceration, after a 3 minute 50-100ppm chlorine rinse or after a 3 minute 2% lactic acid rinse. The rinse fluid was examined for aerobic plate count (APC) on Tryptic Soy Agar (TSA) and total coliforms (TC) on Violet Red Bile Agar. Carcasses sampled immediately after evisceration were examined for the presence of Salmonella, and no Salmonella were detected. Expectedly, chlorine (3.78 log10 cfu/carcass) and lactic acid (2.26 log10 cfu/carcass) APC were significantly (p< 0.01) lower than chlorine (3.78 log10 cfu/carcass). Furthermore, lactic acid TC (<0.30 log10 cfu/carcass estimated count below detection limit) were significantly (p< 0.01) lower than chlorine (2.93 log10 cfu/carcass). Interestingly, there was no significant (p=0.10) difference between chlorine (2.93 log10 cfu/carcass) and no rinse (3.13 log10 cfu/carcass) treatments for TC. Clearly, both the lab and field studies validated the 2% lactic acid rinse as an alternative to 50-100ppm chlorine, providing significant reductions in Salmonella, APC and TC.
Metrics
Details
- Title
- Validation of a 2% lactic acid antimicrobial rinse as an alternative to chlorine for mobile poultry slaughter operations
- Creators
- Aditi Kannan
- Contributors
- Karen Killinger (Degree Supervisor)
- Awarding Institution
- Washington State University
- Academic Unit
- Food Science, School of
- Theses and Dissertations
- Master of Science (MS), Washington State University
- Publisher
- Washington State University; Pullman, Wash. :
- Identifiers
- 99900525179901842
- Language
- English
- Resource Type
- Thesis