Journal article
10 000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresis
Lab on a chip, Vol.11(5), pp.890-898
03/07/2011
Handle:
https://hdl.handle.net/2376/114477
PMID: 21416810
Abstract
This paper describes the preconcentration of the biomarker cardiac troponin I (cTnI) and a fluorescent protein (R-phycoerythrin) using cationic isotachophoresis (ITP) in a 3.9 cm long poly(methyl methacrylate) (PMMA) microfluidic chip. The microfluidic chip includes a channel with a 5× reduction in depth and a 10× reduction in width. Thus, the overall cross-sectional area decreases by 50× from inlet (anode) to outlet (cathode). The concentration is inversely proportional to the cross-sectional area so that as proteins migrate through the reductions, the concentrations increase proportionally. In addition, the proteins gain additional concentration by ITP. We observe that by performing ITP in a cross-sectional area reducing microfluidic chip we can attain concentration factors greater than 10 000. The starting concentration of cTnI was 2.3 μg mL
−1
and the final concentration after ITP concentration in the microfluidic chip was 25.52 ± 1.25 mg mL
−1
. To the author’s knowledge this is the first attempt at concentrating the cardiac biomarker cTnI by ITP. This experimental approach could be coupled to an immunoassay based technique and has the potential to lower limits of detection, increase sensitivity, and quantify different isolated cTnI phosphorylation states.
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Details
- Title
- 10 000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresis
- Creators
- Danny Bottenus - Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, P.O. Box 642710, Pullman, WA, 99164-2710, USA; Fax: +1 335-4806Talukder Zaki Jubery - Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, P.O. Box 642710, Pullman, WA, 99164-2710, USA; Fax: +1 335-4806Yexin Ouyang - Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, P.O. Box 642710, Pullman, WA, 99164-2710, USA; Fax: +1 335-4806Wen-Ji Dong - Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, P.O. Box 642710, Pullman, WA, 99164-2710, USA; Fax: +1 335-4806Prashanta Dutta - Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, P.O. Box 642710, Pullman, WA, 99164-2710, USA; Fax: +1 335-4806Cornelius F Ivory - Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, P.O. Box 642710, Pullman, WA, 99164-2710, USA; Fax: +1 335-4806
- Publication Details
- Lab on a chip, Vol.11(5), pp.890-898
- Academic Unit
- Chemical Engineering and Bioengineering, School of; Mechanical and Materials Engineering, School of
- Grant note
- R01 HL080186-08 || HL / National Heart, Lung, and Blood Institute : NHLBI T32 GM008336-23 || GM / National Institute of General Medical Sciences : NIGMS
- Identifiers
- 99900548319501842
- Language
- English
- Resource Type
- Journal article