Journal article
10000-fold concentration increase in proteins in a cascade microchip using anionic ITP by a 3-D numerical simulation with experimental results
Electrophoresis, Vol.32(5), pp.550-562
02/01/2011
Handle:
https://hdl.handle.net/2376/107367
Abstract
This paper describes both the experimental application and 3-D numerical simulation of isotachophoresis (ITP) in a 3.2cm long 'cascade' poly(methyl methacrylate) (PMMA) microfluidic chip. The microchip includes 10X reductions in both the width and depth of the microchannel, which decreases the overall cross-sectional area by a factor of 100 between the inlet (cathode) and outlet (anode). A 3-D numerical simulation of ITP is outlined and is a first example of an ITP simulation in three dimensions. The 3-D numerical simulation uses COMSOL Multiphysics v4.0a to concentrate two generic proteins and monitor protein migration through the microchannel. In performing an ITP simulation on this microchip platform, we observe an increase in concentration by over a factor of more than 10000 due to the combination of ITP stacking and the reduction in cross-sectional area. Two fluorescent proteins, green fluorescent protein and R-phycoerythrin, were used to experimentally visualize ITP through the fabricated microfluidic chip. The initial concentration of each protein in the sample was 1.995 Delta *mg/mL and, after preconcentration by ITP, the final concentrations of the two fluorescent proteins were 32.57+/-3.63 and 22.81+/-4.61mg/mL, respectively. Thus, experimentally the two fluorescent proteins were concentrated by over a factor of 10000 and show good qualitative agreement with our simulation results.
Metrics
4 Record Views
Details
- Title
- 10000-fold concentration increase in proteins in a cascade microchip using anionic ITP by a 3-D numerical simulation with experimental results
- Creators
- Danny BottenusTalukder Zaki JuberyPrashanta DuttaCornelius F Ivory
- Publication Details
- Electrophoresis, Vol.32(5), pp.550-562
- Academic Unit
- Chemical Engineering and Bioengineering, School of; Mechanical and Materials Engineering, School of
- Identifiers
- 99900547177701842
- Language
- English
- Resource Type
- Journal article