Journal article
3D printed tricalcium phosphate bone tissue engineering scaffolds: effect of SrO and MgO doping on in vivo osteogenesis in a rat distal femoral defect model
Biomaterials science, Vol.1(12), pp.1250-1259
2013
Handle:
https://hdl.handle.net/2376/109499
PMID: 24729867
Abstract
The presence of interconnected macro pores is important in tissue engineering scaffolds for guided tissue regeneration. This study reports in vivo biological performance of interconnected macro porous tricalcium phosphate (TCP) scaffolds due to the addition of SrO and MgO as dopants in TCP. We have used direct three dimensional printing (3DP) technology for scaffold fabrication followed by microwave sintering. Mechanical strength was evaluated for scaffolds with 500 μm, 750 μm, and 1000 μm interconnected designed pore sizes. Maximum compressive strength of 12.01 ± 1.56 MPa was achieved for Sr–Mg doped scaffold with 500 μm interconnected designed pore size. In vivo biological performance of the microwave sintered pure TCP and Sr–Mg doped TCP scaffolds was assessed by implanting 350 μm designed interconnected macro porous scaffolds in rat distal femoral defect. Sintered pore size of these 3D printed scaffolds were 311 ± 5.9 μm and 245 ± 7.5 μm for pure and SrO–MgO doped TCP scaffolds, respectively. These 3D printed scaffolds possessed multiscale porosity, i.e., 3D interconnected designed macro pores along with intrinsic micro pores. Histomorphology and histomorphometric analysis revealed a significant increase in osteoid like new bone formation, and accelerated mineralization inside SrO and MgO doped 3D printed TCP scaffolds as compared to pure TCP scaffolds. An increase in osteocalcin and type I collagen level was also observed in rat blood serum with SrO and MgO doped TCP scaffolds compared to pure TCP scaffolds. Our results show that these 3D printed SrO and MgO doped TCP scaffolds with multiscale porosity contributed to early healing through accelerated osteogenesis.
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Details
- Title
- 3D printed tricalcium phosphate bone tissue engineering scaffolds: effect of SrO and MgO doping on in vivo osteogenesis in a rat distal femoral defect model
- Creators
- Solaiman TarafderNeal M DaviesAmit BandyopadhyaySusmita Bose
- Publication Details
- Biomaterials science, Vol.1(12), pp.1250-1259
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Royal Soc Chemistry
- Number of pages
- 10
- Grant note
- R01EB007351 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB) R01 EB007351 / NIBIB NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)
- Identifiers
- 99900547232901842
- Language
- English
- Resource Type
- Journal article