A 3'-truncated transferrin messenger RNA is expressed in rat testicular germ cells

Steven R Sylvester; Andre Roy

doi:10.1095/biolreprod67.3.895

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A 3'-truncated transferrin messenger RNA is expressed in rat testicular germ cells

Journal article

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A 3'-truncated transferrin messenger RNA is expressed in rat testicular germ cells

Steven R Sylvester and Andre Roy

Biology of reproduction, Vol.67(3), pp.895-899

09/2002

DOI: https://doi.org/10.1095/biolreprod67.3.895

Handle:

https://hdl.handle.net/2376/109699

PMID: 12193400

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Abstract

DNA, Complementary - analysis

Spermatozoa - chemistry

Gene Library

Testis - cytology

Electrophoresis, Polyacrylamide Gel

RNA, Messenger - analysis

Rats

Ribonucleases

Transferrin - genetics

Blotting, Western

Transferrin - analysis

Immunosorbent Techniques

Blotting, Northern

Cattle

Metalloproteins - genetics

RNA, Messenger - chemistry

Peptide Fragments - genetics

Metalloproteins - analysis

Gene Expression

Rat germ cells express a 0.9-kilobase (kb) message with a sequence similar to that of the 3' portion of mammalian transferrins. The sequence of this transcript, called hemiferrin, was considered unique, suggesting that it was encoded by a gene different from that of rat transferrin. Difficulties in conducting experiments using hemiferrin sequence primers led us to question the original sequence. Ribonuclease protection assays revealed that the hemiferrin sequence provided protection only for bovine sequences and not for rat mRNA. Conversely, a 3' rat transferrin sequence protected only rat liver and testis RNA sequences and not bovine sequences, indicating that the 0.9-kb transcript in germ cells is a truncated form of rat transferrin. Western analysis and immunoprecipitation of germ cell proteins metabolically radiolabeled in vitro and in vivo failed to detect a protein of the predicted size regardless of whether anti-rat transferrin or anti-hemiferrin antibodies were used. The findings suggest that a foreshortened transcript of the transferrin gene is produced in rat germ cells and that little or no protein is made from that transcript.

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Title: A 3'-truncated transferrin messenger RNA is expressed in rat testicular germ cells
Creators: Steven R Sylvester - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Vancouver, Washington 98686, USA. sylveste@vancouver.wsu.edu
Andre Roy
Publication Details: Biology of reproduction, Vol.67(3), pp.895-899
Academic Unit: School of Molecular Biosciences
Publisher: United States
Grant note: HD37180 / NICHD NIH HHS
Identifiers: 99900547275301842
Language: English
Resource Type: Journal article