A Babesia bovis 225-kilodalton spherical-body protein: localization to the cytoplasmic face of infected erythrocytes after merozoite invasion

S C Dowling; L E Perryman; D P Jasmer

doi:10.1128/IAI.64.7.2618-2626.1996

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A Babesia bovis 225-kilodalton spherical-body protein: localization to the cytoplasmic face of infected erythrocytes after merozoite invasion

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A Babesia bovis 225-kilodalton spherical-body protein: localization to the cytoplasmic face of infected erythrocytes after merozoite invasion

S C Dowling, L E Perryman and D P Jasmer

Infection and immunity, Vol.64(7), pp.2618-2626

07/1996

DOI: https://doi.org/10.1128/IAI.64.7.2618-2626.1996

Handle:

https://hdl.handle.net/2376/106134

PMCID: PMC174118

PMID: 8698487

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Abstract

Cytoplasm - parasitology

Epitopes - metabolism

Molecular Weight

Erythrocytes - ultrastructure

Molecular Sequence Data

Cytoplasm - metabolism

Cattle Diseases - parasitology

Protozoan Proteins - genetics

Microscopy, Immunoelectron

Antigens, Protozoan - metabolism

Organelles - ultrastructure

Protozoan Proteins - metabolism

Cattle

Protozoan Proteins - chemistry

Antigens, Protozoan - genetics

Amino Acid Sequence

Antigens, Protozoan - chemistry

Antibodies, Monoclonal

Epitopes - genetics

Babesia bovis - metabolism

Babesia bovis - pathogenicity

Animals

Babesiosis - parasitology

Erythrocytes - metabolism

Mice

Babesia bovis - genetics

Erythrocytes - parasitology

In Vitro Techniques

Organelles - metabolism

A 225-kDa Babesia bovis protein occurs on the cytoplasmic side of infected-erythrocyte membranes. Here it is demonstrated that the 225-kDa protein localizes to spherical-body organelles of merozoites. Organelles consistent in size and shape with spherical bodies were isolated between 1.17 and 1.21 g/cm(3) in a sucrose density gradient. Organelles consistent with rhoptries and micronemes were also present in fractions from 1.17 to 1.19 g/cm(3). Antisera generated by immunizing mice with the fraction (1.20 to 1.21 g/cm(3)) most enriched for spherical bodies reacted predominantly with spherical bodies in B. bovis merozoites. A monoclonal antibody generated from this immunization (70/97.14) recognized an epitope that occurs in the repeat region of the 225-kDa protein (now referred to as SBP2). Monoclonal antibody 70/97.14 bound to merozoite spherical bodies, vesicles in infected-host cytoplasm, and the cytoplasmic face of the infected-erythrocyte membrane. These results indicate that spherical-body proteins become associated with the host membrane via transport through the erythrocyte cytoplasm after intracellular invasion.

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Title: A Babesia bovis 225-kilodalton spherical-body protein: localization to the cytoplasmic face of infected erythrocytes after merozoite invasion
Creators: S C Dowling - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7040, USA. dud@vetmed.wsu.edu
L E Perryman
D P Jasmer
Publication Details: Infection and immunity, Vol.64(7), pp.2618-2626
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: AI-01079-01 / NIAID NIH HHS
Identifiers: 99900547120201842
Language: English
Resource Type: Journal article