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CC BY V4.0, Open Access
Journal article
A Calcium/Calmodulin-regulated Member of the Receptor-like Kinase Family Confers Cold Tolerance in Plants
The Journal of biological chemistry, Vol.285(10), pp.7119-7126
03/05/2010
Handle:
https://hdl.handle.net/2376/102919
PMCID: PMC2844161
PMID: 20026608
Abstract
Cold is a limiting environmental factor that adversely affects plant growth and productivity. Calcium/calmodulin-mediated signaling is believed to play a pivotal role in plant response to cold stress, but its exact role is not clearly understood. Here, we report that CRLK1, a novel calcium/calmodulin-regulated receptor-like kinase, is crucial for cold tolerance in plants. CRLK1 has two calmodulin-binding sites with different affinities as follows: one located at residues 369–390 with a
K
d
of 25 n
m
, and the other located at residues 28–112 with a
K
d
of 160 n
m
. Calcium/calmodulin stimulated the kinase activity, but the addition of chlorpromazine, a calmodulin antagonist, blocked its stimulation. CRLK1 is mainly localized in the plasma membrane, and its expression is stimulated by cold and hydrogen peroxide treatments. Under normal growth conditions, there is no noticeable phenotypic difference between wild-type and
crlk1
knock-out mutant plants. However, as compared with wild-type plants, the
crlk1
knock-out mutants exhibited an increased sensitivity to chilling and freezing temperatures. Northern analysis showed that the induction of cold-responsive genes, including
CBF1, RD29A, COR15a
, and
KIN1
in
crlk1
mutants, is delayed as compared with wild-type plants. These results indicate that CRLK1 is a positive regulator of cold tolerance in plants. Furthermore, our results suggest that CRLK1 plays a role in bridging calcium/calmodulin signaling and cold signaling.
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Details
- Title
- A Calcium/Calmodulin-regulated Member of the Receptor-like Kinase Family Confers Cold Tolerance in Plants
- Creators
- Tianbao Yang - From the Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, Washington 99164-6414Shubho Chaudhuri - From the Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, Washington 99164-6414Lihua Yang - From the Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, Washington 99164-6414Liqun Du - From the Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, Washington 99164-6414B. W Poovaiah - From the Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, Washington 99164-6414
- Publication Details
- The Journal of biological chemistry, Vol.285(10), pp.7119-7126
- Academic Unit
- Horticulture, Department of
- Publisher
- American Society for Biochemistry and Molecular Biology; 9650 Rockville Pike, Bethesda, MD 20814, U.S.A
- Identifiers
- 99900546549101842
- Language
- English
- Resource Type
- Journal article