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CC BY V4.0, Open Access
Journal article
A Functional Slow Recycling Pathway of Transferrin is Required for Growth of Chlamydia
Frontiers in microbiology, Vol.1(112 1), pp.112-112
2010
Handle:
https://hdl.handle.net/2376/105260
PMID: 21607082
Abstract
An inhibitor of host cell lysophospholipid acyltransferase, an enzyme involved in lipid metabolism blocked growth of the obligate intracellular pathogen
Chlamydia
through its action on the transport of transferrin (Tf) via the slow pathway of recycling. A detailed characterization of this inhibition revealed that Tf accumulated in vesicles positive for Rab11, with a concomitant reduction in the level of Tf found within the transport intermediate Rab4/11 hybrid vesicles. The net result was the failure to be recycled to the plasma membrane. In chlamydiae-infected cells, the Tf-containing Rab11-positive vesicles were typically found intimately associated with the inclusion, and treatment with the inhibitor caused their accumulation, suggesting that the timely progression and completion of Tf recycling was necessary for proper chlamydial growth. Growth inhibition by the compound could be negated by the simple removal of the Tf-containing fraction of the serum, a further indication that accumulation of Tf around the chlamydial inclusion was deleterious to the pathogen. Thus, it appears that manipulating the slow recycling pathway can have biological consequences for
Chlamydia
and implies the need to regulate carefully the interaction of the inclusion with this host trafficking pathway.
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Details
- Title
- A Functional Slow Recycling Pathway of Transferrin is Required for Growth of Chlamydia
- Creators
- Scot P Ouellette - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College LondonRey A Carabeo - Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London
- Publication Details
- Frontiers in microbiology, Vol.1(112 1), pp.112-112
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Frontiers Research Foundation
- Number of pages
- 12
- Grant note
- R01AI065545 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) G0900213 / MRC; UK Research & Innovation (UKRI); Medical Research Council UK (MRC) AI065545 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA G0900213 / Medical Research Council; UK Research & Innovation (UKRI); Medical Research Council UK (MRC); European Commission
- Identifiers
- 99900546948401842
- Language
- English
- Resource Type
- Journal article