Journal article
A MYB coiled‐coil transcription factor interacts with NSP2 and is involved in nodulation in Lotus japonicus
The New phytologist, Vol.201(3), pp.837-849
02/2014
Handle:
https://hdl.handle.net/2376/105906
PMID: 24400899
Abstract
Summary
Transcription factor complex formation is a central step in regulating gene expression. In this report, a novel MYB coiled‐coil transcription factor referred to as IPN2, for Interacting Protein of NSP2, is described.
The interaction between IPN2 and NSP2 was examined by protein pull‐down assays and bimolecular fluorescence complementation (BiFC). Subcellular localization of proteins, gene expression and gene function were assessed in transgenic hairy roots expressing tagged recombinant proteins, promoter‐reporter and RNA interference (RNAi) constructs, respectively.
The GRAS domain of NSP2 and the coiled‐coil domain of IPN2 were found to be responsible for the interaction between the two proteins. IPN2 had strong transcription activation activity, bound directly to the NIN gene promoter, and was localized to the nuclei of Lotus japonicus root cells. The expression of IPN2 was elevated during nodule development, coinciding with increased NSP2 gene expression during nodule organogenesis. RNAi‐mediated knockdown expression of IPN2 did not affect arbuscular mycorrhizal development, but had deleterious effects on rhizobial infection and nodule formation in L. japonicus.
These results demonstrate an important role of IPN2 in nodule organogenesis and place a new MYB transcription factor in the Nod signaling pathway.
Metrics
12 Record Views
Details
- Title
- A MYB coiled‐coil transcription factor interacts with NSP2 and is involved in nodulation in Lotus japonicus
- Creators
- Heng Kang - Huazhong Agricultural UniversityXiaojie Chu - Huazhong Agricultural UniversityChao Wang - Huazhong Agricultural UniversityAifang Xiao - Huazhong Agricultural UniversityHui Zhu - Huazhong Agricultural UniversitySongli Yuan - Huazhong Agricultural UniversityZhenzhen Yang - Huazhong Agricultural UniversityDanxia Ke - Huazhong Agricultural UniversityShaobo Xiao - Huazhong Agricultural UniversityZonglie Hong - University of IdahoZhongming Zhang - Huazhong Agricultural University
- Publication Details
- The New phytologist, Vol.201(3), pp.837-849
- Academic Unit
- Center for Reproductive Biology; Crop and Soil Sciences, Department of
- Number of pages
- 13
- Grant note
- National Natural Science Foundation of China (30900096; 31170224; 31200188) China Postdoctoral Science Foundation (2012M521436; 2013T60727) National Basic Research Program of China (2010CB126502)
- Identifiers
- 99900546551101842
- Language
- English
- Resource Type
- Journal article