Journal article
A Model of Cytokine Shedding Induced by Low Doses of Gamma Radiation
Radiation research, Vol.163(3), pp.337-342
03/01/2005
Handle:
https://hdl.handle.net/2376/115725
Abstract
Miller, J. H., Zheng, F., Jin, S., Opresko, L. K., Wiley, H. S. and Resat, H. A Model of Cytokine Shedding Induced by Low Doses of Gamma Radiation. Radiat. Res. 163, 337- 342 (2005).A model for sustained shedding of epidermal growth factor (EGF) in response to low doses of gamma radiation was developed based on a time delay in the feedback from mitogen-activated protein kinase (MAPK) activation to metalloprotease activity in an autocrine signaling process. We determined the kinetic parameters of our model using the data available for MAPK activation by gamma irradiation in the 1-2-Gy dose range and then showed that predictions of the model were consistent with experimental results for the kinetics of EGF shedding into the growth medium after exposure of human mammary epithelial cells to 1-5 cGy of gamma radiation in the presence of antibodies that block ligand binding to EGF receptors. The model allowed us to estimate the rate of radiation-induced cytokine release per cell from measurements of EGF concentration in the growth medium and to assess the effectiveness of EGF shedding and subsequent diffusion through the medium as a mechanism for signal transmission between hit cells and bystanders.
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Details
- Title
- A Model of Cytokine Shedding Induced by Low Doses of Gamma Radiation
- Creators
- J H MillerF ZhengS JinL K OpreskoH S WileyH Resat
- Publication Details
- Radiation research, Vol.163(3), pp.337-342
- Academic Unit
- Engineering and Applied Sciences (TRIC), School of
- Identifiers
- 99900547613501842
- Language
- English
- Resource Type
- Journal article