Journal article
A Multivalent Mannheimia-Bibersteinia Vaccine Protects Bighorn Sheep against Mannheimia haemolytica Challenge
Clinical and vaccine immunology, Vol.18(10), pp.1689-1694
10/2011
Handle:
https://hdl.handle.net/2376/114569
PMCID: PMC3187036
PMID: 21832104
Abstract
Bighorn sheep (BHS) are more susceptible than domestic sheep (DS) to Mannheimia haemolytica pneumonia. Although both species carry M. haemolytica as a commensal bacterium in the nasopharynx, DS carry mostly leukotoxin (Lkt)-positive strains while BHS carry Lkt-negative strains. Consequently, antibodies to surface antigens and Lkt are present at much higher titers in DS than in BHS. The objective of this study was to determine whether repeated immunization of BHS with multivalent Mannheimia - Bibersteinia vaccine will protect them upon M. haemolytica challenge. Four BHS were vaccinated with a culture supernatant vaccine prepared from M. haemolytica serotypes A1 and A2 and Bibersteinia trehalosi serotype T10 on days 0, 21, 35, 49, and 77. Four other BHS were used as nonvaccinated controls. On the day of challenge, 12 days after the last immunization, the mean serum titers of Lkt-neutralizing antibodies and antibodies to surface antigens against M. haemolytica were 1:160 and 1:4,000, respectively. Following intranasal challenge with M. haemolytica A2 (1 × 10 5 CFU), all four control BHS died within 48 h. Necropsy revealed acute fibrinonecrotic pneumonia characteristic of M. haemolytica infection. None of the vaccinated BHS died during the 8 weeks post challenge observation period. Radiography at 3 weeks post challenge revealed no lung lesions in two vaccinated BHS and mild lesions in the other two, which resolved by 8 weeks postchallenge. These results indicate that if BHS can be induced to develop high titers of Lkt-neutralizing antibodies and antibodies to surface antigens, they are likely to survive M. haemolytica challenge which is likely to reduce the BHS population decline due to pneumonia.
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Details
- Title
- A Multivalent Mannheimia-Bibersteinia Vaccine Protects Bighorn Sheep against Mannheimia haemolytica Challenge
- Creators
- Renuka Subramaniam - Department of Veterinary Microbiology and PathologySudarvili Shanthalingam - Department of Veterinary Microbiology and PathologyJegarubee Bavananthasivam - Department of Veterinary Microbiology and PathologyAbirami Kugadas - Department of Veterinary Microbiology and PathologyKathleen A Potter - Department of Veterinary Microbiology and PathologyWilliam J Foreyt - Department of Veterinary Microbiology and PathologyDouglas C Hodgins - Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, CanadaPatricia E Shewen - Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, CanadaGeorge M Barrington - Department of Veterinary Clinical Medicine and Surgery, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7040Donald P Knowles - Department of Veterinary Microbiology and PathologySubramaniam Srikumaran - Department of Veterinary Microbiology and Pathology
- Publication Details
- Clinical and vaccine immunology, Vol.18(10), pp.1689-1694
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Number of pages
- 6
- Identifiers
- 99900547587901842
- Language
- English
- Resource Type
- Journal article