Journal article
A Novel Nanoparticle-Based Disposable Electrochemical Immunosensor for Diagnosis of Exposure to Toxic Organophosphorus Agents
Advanced functional materials, Vol.21(22), pp.4371-4378
11/22/2011
Abstract
A novel disposable electrochemical immunosensor for highly selective and sensitive detection of organophosphorylated butyrylcholinesterase (OP-BChE), a specific biomarker for exposure to toxic organophosphorus agents, is presented. In this new approach, zirconia nanoparticles (ZrO2) were employed to selectively capture the OP moiety of OP-BChE adducts, followed by quantum dot (QD)-tagged anti-BChE antibodies for amplified quantification. The captured CdSe-QD tags can be sensitively detected by stripping voltammetry using an in situ bismuth-plating method. The OP agent, diisopropylfluorophosphate (DFP), was selected to prepare OP-BChE adducts in various matrices. The formation of OP-BChE adducts in plasma sample was confirmed using mass spectroscopy. The developed electrochemical immunosensor demonstrates a highly linear voltammetric response over the range of 0.1 to 30 nM OP-BChE, with a detection limit of 0.03 nM (based on signal/noise = 3), coupled with a good reproducibility (relative standard deviation 4.5%). Moreover, the immunosensor has been validated with biomonitoring of OP-BChE adducts in the plasma samples. This novel nanoparticle-based electrochemical immunosensor thus provides an alternative way for designing a sensitive and cost-effective sensing platform for on-site screening/evaluating exposure to a variety of OP agents.
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Details
- Title
- A Novel Nanoparticle-Based Disposable Electrochemical Immunosensor for Diagnosis of Exposure to Toxic Organophosphorus Agents
- Creators
- Donglai Lu - Pacific Northwest National LaboratoryJun Wang - Pacific Northwest National LaboratoryLimin Wang - Pacific Northwest National LaboratoryDan Du - Pacific Northwest National LaboratoryCharles Timchalk - Pacific Northwest National LaboratoryRichard Barry - Pacific Northwest National LaboratoryYuehe Lin - Pacific Northwest National Laboratory
- Publication Details
- Advanced functional materials, Vol.21(22), pp.4371-4378
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- U01 NS058161-01 / Office of the Director, National Institutes of Health (OD); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA U.S. Department of Energy (DOE); United States Department of Energy (DOE) National Institute of Neurological Disorders and Stroke (NINDS); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) DE-AC05-76RL01830 / DOE; United States Department of Energy (DOE)
- Identifiers
- 99901227843601842
- Language
- English
- Resource Type
- Journal article