Journal article
A Novel Superfamily of Transporters for Allantoin and Other Oxo Derivatives of Nitrogen Heterocyclic Compounds in Arabidopsis
The Plant cell, Vol.14(4), pp.847-856
04/2002
Handle:
https://hdl.handle.net/2376/116300
PMCID: PMC150686
PMID: 11971139
Abstract
A wide spectrum of soil heterocyclic nitrogen compounds are potential nutrients for plants. Here, it is shown that Arabidopsis plants are able to use allantoin as sole nitrogen source. By functional complementation of a yeast mutant defective in allantoin uptake, an Arabidopsis transporter, AtUPS1 (
Arabidopsis thaliana ureide permease 1
), was identified. AtUPS1 belongs to a novel superfamily of plant membrane proteins with five open reading frames in Arabidopsis (identity, 64 to 82%). UPS proteins have 10 putative transmembrane domains with a large cytosolic central domain containing a “Walker A” motif. Transport of
14
C-labeled allantoin by AtUPS1 in yeast exhibited saturation kinetics (
K
m
∼ 52 μM), was dependent on Glc and a proton gradient, and was stimulated by acidic pH. AtUPS1 transports uric acid and xanthine, besides allantoin, but not adenine. Protons are cosubstrates in allantoin transport by AtUPS1, as demonstrated by expression in
Xenopus laevis
oocytes. In plants, AtUPS1 gene expression was dependent on the nitrogen source. Therefore, AtUPS1 presumably is involved in the uptake of allantoin and other purine degradation products when primary sources are limiting.
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Details
- Title
- A Novel Superfamily of Transporters for Allantoin and Other Oxo Derivatives of Nitrogen Heterocyclic Compounds in Arabidopsis
- Creators
- Marcelo Desimone - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, GermanyElisabetta Catoni - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, GermanyUwe Ludewig - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, GermanyMelanie Hilpert - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, GermanyAnja Schneider - Botany Institute II, University of Cologne, Gyrhofstrasse 15, D-50931 Köln, GermanyReinhard Kunze - Botany Institute II, University of Cologne, Gyrhofstrasse 15, D-50931 Köln, GermanyMechthild Tegeder - School for Biological Sciences, Washington State University, Pullman, Washington 99164–4236Wolf Bernd Frommer - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, GermanyKarin Schumacher - Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
- Publication Details
- The Plant cell, Vol.14(4), pp.847-856
- Academic Unit
- Biological Sciences, School of
- Publisher
- American Society of Plant Biologists
- Number of pages
- 10
- Identifiers
- 99900547340301842
- Language
- English
- Resource Type
- Journal article