Journal article
A PCR-based method of identifying species-specific repeated DNAs
BioTechniques, Vol.13(2), pp.210-214
08/1992
Handle:
https://hdl.handle.net/2376/111645
PMID: 1389154
Abstract
A PCR-based method is described to facilitate the identification of DNA fragments that are highly repeated and species-specific. The precision of the technique was demonstrated by cloning a fragment that occurred in a high number of copies in the plant species Medicago granadensis but in a low number of copies in 17 other Medicago species and Melilotus officinalis. This method should greatly accelerate the isolation and cloning of short and long interspersed nuclear elements with species-specific distributions. Such clones should prove useful in studies of phylogenetic relationships in the identification of interspecific hybrids, as in situ chromosome markers and other applications.
Metrics
6 Record Views
Details
- Title
- A PCR-based method of identifying species-specific repeated DNAs
- Creators
- D Z Skinner - USDA-ARS, Dept. of Agronomy, Kansas State University, Manhattan 66506-5501
- Publication Details
- BioTechniques, Vol.13(2), pp.210-214
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- England
- Identifiers
- 99900547706401842
- Language
- English
- Resource Type
- Journal article