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CC BY V4.0, Open Access
Journal article
A Ubiquitin Ligase of Symbiosis Receptor Kinase Involved in Nodule Organogenesis
Plant physiology (Bethesda), Vol.160(1), pp.106-117
09/2012
Handle:
https://hdl.handle.net/2376/113262
PMID: 22822209
Abstract
The symbiosis receptor kinase (SymRK) is required for morphological changes of legume root hairs triggered by rhizobial infection. How protein turnover of SymRK is regulated and how the nodulation factor signals are transduced downstream of SymRK are not known. In this report, a SymRK-interacting E3 ubiquitin ligase (SIE3) was shown to bind and ubiquitinate SymRK. The SIE3-SymRK interaction and the ubiquitination of SymRK were shown to occur in vitro and in planta. SIE3 represents a new class of plant-specific E3 ligases that contain a unique pattern of the conserved CTLH (for C-terminal to LisH), CRA (for CT11-RanBPM), and RING (for Really Interesting New Gene) domains. Expression of SIE3 was detected in all tested tissues of Lotus japonicus plants, and its transcript level in roots was enhanced by rhizobial infection. The SIE3 protein was localized to multiple subcellular locations including the nuclei and plasma membrane, where the SIE3-SymRK interaction took place. Overexpression of SIE3 promoted nodulation in transgenic hairy roots, whereas downregulation of SIE3 transcripts by RNA interference inhibited infection thread development and nodule organogenesis. These results suggest that SIE3 represents a new class of E3 ubiquitin ligase, acts as a regulator of SymRK, and is involved in rhizobial infection and nodulation in L. japonicus.
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Details
- Title
- A Ubiquitin Ligase of Symbiosis Receptor Kinase Involved in Nodule Organogenesis
- Creators
- Songli Yuan - Huazhong Agricultural UniversityHui Zhu - Huazhong Agricultural UniversityHonglan GouWeiwei Fu - Huazhong Agricultural UniversityLijing Liu - Huazhong Agricultural UniversityTao Chen - Huazhong Agricultural UniversityDanxia Ke - Huazhong Agricultural UniversityHeng Kang - Huazhong Agricultural UniversityQi Xie - Huazhong Agricultural UniversityZonglie Hong - Huazhong Agricultural UniversityZhongming Zhang - Huazhong Agricultural University
- Publication Details
- Plant physiology (Bethesda), Vol.160(1), pp.106-117
- Academic Unit
- Center for Reproductive Biology
- Publisher
- Amer Soc Plant Biologists
- Number of pages
- 12
- Grant note
- Graduate Education Innovation Fund of Huazhong Agricultural University AMLKF200804 / State Key Laboratory of Agricultural Microbiology 170224 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 2010CB126502 / National Basic Research Program of China (973 program); National Basic Research Program of China 2009ZX08009-116B / Ministry of Agriculture of China; Ministry of Agriculture & Rural Affairs
- Identifiers
- 99900547964901842
- Language
- English
- Resource Type
- Journal article