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CC BY V4.0, Open Access
Journal article
A basic domain in the histone H2B N-terminal tail is important for nucleosome assembly by FACT
Nucleic acids research, Vol.44(19), pp.9142-9152
11/02/2016
Handle:
https://hdl.handle.net/2376/112728
PMCID: PMC5100577
PMID: 27369377
Abstract
Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcription elongation and DNA replication. Recent studies have identified candidate histone residues that mediate FACT binding to histones, but it is not known which histone residues are important for FACT to deposit histones onto DNA during nucleosome assembly. In this study, we report that the histone H2B repression (HBR) domain within the H2B N-terminal tail is important for histone deposition by FACT. Deletion of the HBR domain causes significant defects in histone occupancy in the yeast genome, particularly at HBR-repressed genes, and a pronounced increase in H2A-H2B dimers that remain bound to FACT in vivo Moreover, the HBR domain is required for purified FACT to efficiently assemble recombinant nucleosomes in vitro We propose that the interaction between the highly basic HBR domain and DNA plays an important role in stabilizing the nascent nucleosome during the process of histone H2A-H2B deposition by FACT.
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Details
- Title
- A basic domain in the histone H2B N-terminal tail is important for nucleosome assembly by FACT
- Creators
- Peng Mao - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USAMcKenna N M Kyriss - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USAAmelia J Hodges - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USAMingrui Duan - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USARobert T Morris - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USAMark D Lavine - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USATraci B Topping - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USALisa M Gloss - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USAJohn J Wyrick - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USA jwyrick@wsu.edu
- Publication Details
- Nucleic acids research, Vol.44(19), pp.9142-9152
- Academic Unit
- Entomology, Department of; Graduate School; Molecular Biosciences, School of
- Publisher
- England
- Grant note
- T32 GM008336 / NIGMS NIH HHS
- Identifiers
- 99900548349901842
- Language
- English
- Resource Type
- Journal article