Journal article
A brain-specific cytochrome P450 responsible for the majority of deltamethrin resistance in the QTC279 strain of Tribolium castaneum
Proceedings of the National Academy of Sciences - PNAS, Vol.107(19), pp.8557-8562
05/11/2010
Handle:
https://hdl.handle.net/2376/116922
PMCID: PMC2889294
PMID: 20410462
Abstract
Cytochrome P450-mediated detoxification is one of the most important mechanisms involved in insecticide resistance. However, the molecular basis of this mechanism and the physiological functions of P450s associated with insecticide resistance remain largely unknown. Here, we exploited the functional genomics and reverse genetic approaches to identify and characterize a P450 gene responsible for the majority of deltamethrin resistance observed in the QTC279 strain of
Tribolium castaneum
. We used recently completed whole-genome sequence of
T. castaneum
to prepare custom microarrays and identified a P450 gene,
CYP6BQ9
, which showed more than a 200-fold higher expression in the deltamethrin-resistant QTC279 strain when compared with its expression in the deltamethrin-susceptible Lab-S strain. Functional studies using both double-strand RNA (dsRNA)-mediated knockdown in the expression of
CYP6BQ9
and transgenic expression of
CYP6BQ9
in
Drosophila melanogaster
showed that
CYP6BQ9
confers deltamethrin resistance. Furthermore, CYP6BQ9 enzyme expressed in baculovirus metabolizes deltamethrin to 4-hydroxy deltamethrin. Strikingly, we also found that unlike many P450 genes involved in insecticide resistance that were reported previously,
CYP6BQ9
is predominantly expressed in the brain, a part of the central nervous system (CNS) containing voltage-gated sodium channels targeted by deltamethrin. Taken together, the current studies on the brain-specific insect P450 involved in deltamethrin resistance shed new light on the understanding of the molecular basis and evolution of insecticide resistance.
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Details
- Title
- A brain-specific cytochrome P450 responsible for the majority of deltamethrin resistance in the QTC279 strain of Tribolium castaneum
- Creators
- Fang Zhu - Department of Entomology, College of AgricultureR Parthasarathy - Department of Entomology, College of AgricultureHua Bai - Department of Entomology, College of AgricultureKatharina Woithe - , D40789 MonheimMartin Kaussmann - , D40789 MonheimRalf Nauen - , D40789 MonheimDouglas A Harrison - Department of Biology, College of Arts and SciencesSubba R Palli - Department of Entomology, College of Agriculture
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.107(19), pp.8557-8562
- Academic Unit
- School of Biological Sciences
- Publisher
- National Academy of Sciences
- Number of pages
- 6
- Grant note
- NBRE-P20 RR016481; COBRE-P20RR018733 / National Center for Research Resources Institutional Development Award (IDeA); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) P20RR018733 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) 2007-04636 / US Department of Agriculture National Research Initiative Cooperative State Research, Education, and Extension Service; United States Department of Agriculture (USDA); National Institute of Food and Agriculture
- Identifiers
- 99900547768301842
- Language
- English
- Resource Type
- Journal article