A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants

Tianbao Yang; B W Poovaiah

doi:10.1074/jbc.M207941200

Back

A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants

Journal article

Open access

Peer reviewed

A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants

Tianbao Yang and B W Poovaiah

The Journal of biological chemistry, Vol.277(47), pp.45049-45058

11/22/2002

DOI: https://doi.org/10.1074/jbc.M207941200

Handle:

https://hdl.handle.net/2376/114774

PMID: 12218065

Files and links (2)

pdf

PIIS00219258197160442.14 MBDownload View

CC BY V4.0, Open Access

url

https://doi.org/10.1074/jbc.M207941200View

Published (Version of record) Open

Abstract

Protein Kinases - metabolism

Oxidants - pharmacology

Protein Kinases - genetics

Calcium - metabolism

Genes, Plant

Protein-Serine-Threonine Kinases

Molecular Sequence Data

Recombinant Fusion Proteins - metabolism

Arabidopsis Proteins - metabolism

DNA-Binding Proteins - metabolism

Tissue Distribution

Salicylic Acid - pharmacology

Base Sequence

Plant Proteins - metabolism

Calmodulin-Binding Proteins - genetics

Protein Structure, Tertiary

Amino Acid Sequence

Arabidopsis Proteins - genetics

Arabidopsis - drug effects

Calmodulin - metabolism

Hydrogen Peroxide - pharmacology

Calmodulin-Binding Proteins - metabolism

DNA-Binding Proteins - genetics

Arabidopsis - metabolism

Arabidopsis - genetics

Plant Proteins - genetics

Sequence Alignment

Recombinant Fusion Proteins - genetics

Signal Transduction - physiology

Plant Growth Regulators - pharmacology

We reported earlier that the tobacco early ethylene-responsive gene NtER1 encodes a calmodulin-binding protein (Yang, T., and Poovaiah, B. W. (2000) J. Biol. Chem. 275, 38467-38473). Here we demonstrate that there is one NtER1 homolog as well as five related genes in Arabidopsis. These six genes are rapidly and differentially induced by environmental signals such as temperature extremes, UVB, salt, and wounding; hormones such as ethylene and abscisic acid; and signal molecules such as methyl jasmonate, H(2)O(2), and salicylic acid. Hence, they were designated as AtSR1-6 (Arabidopsis thaliana signal-responsive genes). Ca(2+)/calmodulin binds to all AtSRs, and their calmodulin-binding regions are located on a conserved basic amphiphilic alpha-helical motif in the C terminus. AtSR1 targets the nucleus and specifically recognizes a novel 6-bp CGCG box (A/C/G)CGCG(G/T/C). The multiple CGCG cis-elements are found in promoters of genes such as those involved in ethylene signaling, abscisic acid signaling, and light signal perception. The DNA-binding domain in AtSR1 is located on the N-terminal 146 bp where all AtSR1-related proteins share high similarity but have no similarity to other known DNA-binding proteins. The calmodulin-binding nuclear proteins isolated from wounded leaves exhibit specific CGCG box DNA binding activities. These results suggest that the AtSR gene family encodes a family of calmodulin-binding/DNA-binding proteins involved in multiple signal transduction pathways in plants.

Metrics

15 Record Views

See more details

Details

Title: A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants
Creators: Tianbao Yang - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, Washington 99164-6414, USA
B W Poovaiah
Publication Details: The Journal of biological chemistry, Vol.277(47), pp.45049-45058
Academic Unit: Department of Horticulture
Publisher: United States
Identifiers: 99900547805501842
Language: English
Resource Type: Journal article