A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast

Ronita Nag; McKenna Kyriss; John W Smerdon; John J Wyrick; Michael J Smerdon

doi:10.1093/nar/gkp1074

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A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast

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A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast

Ronita Nag, McKenna Kyriss, John W Smerdon, John J Wyrick and Michael J Smerdon

Nucleic acids research, Vol.38(5), pp.1450-1460

03/2010

DOI: https://doi.org/10.1093/nar/gkp1074

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https://hdl.handle.net/2376/109903

PMCID: PMC2836547

PMID: 20007597

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Abstract

Genome Integrity, Repair and

The highly charged histone N-terminal domains are engaged in inter- and intra-nucleosomal interactions, and contain a host of sites used for posttranslational modification. We have studied the effect of deleting residues 30–37 from the N-terminal domain of histone H2B in yeast cells, on nucleotide excision repair (NER) following UV irradiation, as these cells are quite sensitive to UV. We find that H2B Δ30–37 cells exhibit reduced NER efficiency at three specific chromatin loci: the transcriptionally active, RPB2 locus; the transcriptionally silenced, nucleosome-loaded HML locus; and the transcriptionally repressed, non-silenced, GAL10 locus. Nuclease digestion studies indicate that H2B Δ30–37 chromatin has increased nucleosome accessibility and/or nucleosome mobility. In addition, H2B Δ30–37 mutants acquire more DNA damage, compared to wt cells, following the same dose of UV radiation. Reducing the level of damage in H2B Δ30–37 cells to match that of wt cells restores the NER rate to wt levels in the RPB2 and GAL10 loci, but NER efficiency remains low in the silenced HML locus. Interestingly, recruitment of Snf5 to the HML locus is reduced in H2B Δ30–37 cells and more transient following UV irradiation. This may reflect a lower binding affinity of the SWI/SNF complex to H2B Δ30–37 nucleosomes.

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Title: A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast
Creators: Ronita Nag - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, USA
McKenna Kyriss - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, USA
John W Smerdon - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, USA
John J Wyrick - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, USA
Michael J Smerdon - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, USA
Publication Details: Nucleic acids research, Vol.38(5), pp.1450-1460
Academic Unit: School of Molecular Biosciences
Publisher: Oxford University Press
Identifiers: 99900547730401842
Language: English
Resource Type: Journal article