Journal article
A central role for ceramide in the age‐related acceleration of apoptosis in the female germline
The FASEB journal, Vol.19(7), pp.1-23
05/2005
Handle:
https://hdl.handle.net/2376/110310
PMID: 15728664
Abstract
ABSTRACT
An age‐dependent acceleration of apoptosis occurs in female germ cells (oocytes), and this requires communication between the oocyte and its surrounding somatic (cumulus) cells. Here we show in aged mice that ceramide is translocated from cumulus cells into the adjacent oocyte and induces germ cell apoptosis that can be prevented by sphingosine‐1‐phosphate. Trafficking of ceramide requires gap junction‐dependent communication between the cumulus cells and the oocyte as well as intact lipid rafts. Further, the occurrence of the elevated incidence of apoptosis in oocytes of aged females is concomitant with an enhanced sensitivity of the oocyte to a spike in cytosolic ceramide levels, as well as increased bax mRNA and Bax protein levels. Thus, the force driving the age‐related increase in female germ cell death is multifactorial, but changes in the intercellular trafficking of ceramide, along with hypersensitivity of oocytes to ceramide, are key factors in this process.
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Details
- Title
- A central role for ceramide in the age‐related acceleration of apoptosis in the female germline
- Creators
- Gloria I Perez - Massachusetts General Hospital/Harvard Medical SchoolAndrea Jurisicova - University of TorontoTiina Matikainen - Massachusetts General Hospital/Harvard Medical SchoolToshitake Moriyama - Massachusetts General Hospital/Harvard Medical SchoolMee-Ran Kim - Massachusetts General Hospital/Harvard Medical SchoolYasushi Takai - Massachusetts General Hospital/Harvard Medical SchoolJames K Pru - Massachusetts General Hospital/Harvard Medical SchoolRichard N Kolesnick - Memorial Sloan-Kettering Cancer CenterJonathan L Tilly - Massachusetts General Hospital/Harvard Medical School
- Publication Details
- The FASEB journal, Vol.19(7), pp.1-23
- Academic Unit
- Animal Sciences, Department of
- Number of pages
- 23
- Grant note
- National Institutes of Health (R01-AG12279) Kobe University School of Medicine The Catholic University of Korea Canadian Institutes of Health Finnish Cultural Foundation
- Identifiers
- 99900547311701842
- Language
- English
- Resource Type
- Journal article