Journal article
A cloned gene of Cryptosporidium parvum encodes neutralization-sensitive epitopes
Molecular and biochemical parasitology, Vol.80(2), pp.137-147
1996
Handle:
https://hdl.handle.net/2376/109259
PMID: 8892291
Abstract
Two mAb, C6B6 and 7D10, each significantly reduced infection of mice by
Cryptosporidium parvum and reacted with a 23-kDa glycoprotein (p23) of geographically disperse
C. parvum isolates. The antibodies were used to identify plaques in a cDNA library prepared from
C. parvum sporozoite mRNA. cDNA insert sequences from positive plaques were determined and used to isolate additional clones encoding p23 coding sequences. A consensus open reading frame of 333 base pairs, encoding 111 amino acids, was identified in this collection of cDNAs. The predicted amino acid sequence contained one N-glycosylation site, but lacked hydrophobic membrane spanning regions. Epitope mapping revealed that mAb 7D10 defines the linear epitope QDKPAD which occurs twice in the C terminal region of the peptide encoded by the ORF. This same C terminal peptide region contains a non-linear epitope bound by mAb C6B6. Serum from mice immunized with synthetic C terminal peptide reacted with sporozoite p23. The occurrence of neutralization-sensitive epitopes encoded by defined regions of the
C. parvum genome suggests that recombinant proteins or synthetic peptides containing these epitopes may prove useful for inducing immune responses that diminish infection.
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Details
- Title
- A cloned gene of Cryptosporidium parvum encodes neutralization-sensitive epitopes
- Creators
- Lance E Perryman - Department of Microbiology, Pathology, and Parasitology, North Carolina State University, 4700 Hillsborough Street, Raleigh NC 27606, USADouglas P Jasmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman WA 99164-7040, USAMichael W Riggs - Department of Verterinary Science, University of Arizona, Tucson AZ 85721, USAStewart G Bohnet - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman WA 99164-7040, USATravis C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman WA 99164-7040, USAMichael J Arrowood - Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta GA 30341-3724, USA
- Publication Details
- Molecular and biochemical parasitology, Vol.80(2), pp.137-147
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547735201842
- Language
- English
- Resource Type
- Journal article