A common core for binding single-stranded DNA: structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria

Gordon Webster; Jochen Genschel; Ute Curth; Claus Urbanke; ChulHee Kang; Rolf Hilgenfeld

doi:10.1016/S0014-5793(97)00747-3

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A common core for binding single-stranded DNA: structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria

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A common core for binding single-stranded DNA: structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria

Gordon Webster, Jochen Genschel, Ute Curth, Claus Urbanke, ChulHee Kang and Rolf Hilgenfeld

FEBS letters, Vol.411(2), pp.313-316

1997

DOI: https://doi.org/10.1016/S0014-5793(97)00747-3

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https://hdl.handle.net/2376/108655

PMID: 9271227

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Abstract

Single-stranded DNA-binding protein

SSB

Crystal structure

The crystal structure of the DNA-binding domain of E. coli SSB ( EcoSSB) has been determined to a resolution of 2.5 Å. This is the first reported structure of a prokaryotic SSB. The structure of the DNA-binding domain of the E. coli protein is compared to that of the human mitochondrial SSB ( HsmtSSB). In spite of the relatively low sequence identity between them, the two proteins display a high degree of structural similarity. EcoSSB crystallises with two dimers in the asymmetric unit, unlike HsmtSSB which contains only a dimer. This is probably a consequence of the different polypeptide chain lengths in the EcoSSB heterotetramer. Crucial differences in the dimer-dimer interface of EcoSSB may account for the inability of EcoSSB and HsmtSSB to form cross-species heterotetramers, in contrast to many bacterial SSBs.

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Title: A common core for binding single-stranded DNA: structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria
Creators: Gordon Webster - Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany
Jochen Genschel - Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany
Ute Curth - Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany
Claus Urbanke - Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany
ChulHee Kang - Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany
Rolf Hilgenfeld
Publication Details: FEBS letters, Vol.411(2), pp.313-316
Academic Unit: Department of Chemistry
Publisher: Elsevier B.V
Number of pages: 4
Identifiers: 99900546759101842
Language: English
Resource Type: Journal article