Journal article
A disposable sensor based on immobilization of acetylcholinesterase to multiwall carbon nanotube modified screen-printed electrode for determination of carbaryl
Journal of applied electrochemistry, Vol.38(9), pp.1217-1222
09/2008
Handle:
https://hdl.handle.net/2376/114874
Abstract
A simple method has been devised for immobilization of acetylcholinesterase (AChE) covalent bonding to a multiwall carbon nanotube (MWNT)-cross-linked cellulose acetate composite on a screen-printed carbon electrode (SPCE) and a sensitive and disposable amperometric sensor for rapid determination of carbaryl pesticide is proposed. The immobilized enzyme was preserved on this film because of the excellent biocompatibility and non-toxicity of cellulose acetate. Based on the inherent conductive properties of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl. MWNT improved the interface enzymatic hydrolysis reaction and increased the amperometric response of the sensor. Under optimum conditions, the inhibition of carbaryl on AChE increased linearly with the increasing concentration of carbaryl in two ranges, from 0.01 to 0.5 μg mL−1 and from 2 to 20 μg mL−1, with the correlation coefficients of 0.9985 and 0.9977, respectively. The detection limit was 0.004 μg mL−1 taken as the concentration equivalent to 10% decrease in signal. The sensor showed acceptable stability, accuracy and could be fabricated in batches, thus it is economic and portable. This type of disposable enzyme-based amperometric sensor has extensive application potential in environmental monitoring of pesticides.
Metrics
17 Record Views
Details
- Title
- A disposable sensor based on immobilization of acetylcholinesterase to multiwall carbon nanotube modified screen-printed electrode for determination of carbaryl
- Creators
- Jie Cai - The Technology Center of Wuhan Iron and Steel Company Wuhan 430080 P.R. ChinaDan Du - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry Central China Normal University Wuhan 430079 P.R. China
- Publication Details
- Journal of applied electrochemistry, Vol.38(9), pp.1217-1222
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900548182601842
- Language
- English
- Resource Type
- Journal article