Journal article
A flow cytometric method for assessing viability of intraerythrocytic hemoparasites
Journal of immunological methods, Vol.140(1), pp.23-30
1991
Handle:
https://hdl.handle.net/2376/104440
PMID: 2061611
Abstract
We have developed a rapid, reliable method of evaluating growth and viability of intraerythrocytic protozoan hemoparasites. The assay involves the selective uptake and metabolic conversion of hydroethidine to ethidium by live parasites present in intact erythrocytes. The red fluorescence imparted by ethidium intercalated into the DNA of the parasite permits the use of flow cytometry to distinguish infected erythrocytes with viable parasites from uninfected erythrocytes and erythrocytes containing dead parasites. Comparison of the fluorochromasia technique of enumerating the number and viability of hemoparasites in cultured erythrocytes with enumeration in Giemsa-stained films and uptake of [
3H]hypoxanthine demonstrated the fluorochromasia technique yields comparable results. Studies with the hemoparasite,
Babesia bovis, have shown the fluorochromasia technique can also be used to monitor the effect of parasiticidal drugs on parasites in vitro. The cumulative studies with the fluorochromasia assay suggest the assay will also prove useful in investigations focused on analysis of the immune response to hemoparasites and growth in vitro.
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Details
- Title
- A flow cytometric method for assessing viability of intraerythrocytic hemoparasites
- Creators
- Carol R Wyatt - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, U.S.AWill Goff - USDA, ARS, Pullman, WA 99164, U.S.AWilliam C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, U.S.A
- Publication Details
- Journal of immunological methods, Vol.140(1), pp.23-30
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546806301842
- Language
- English
- Resource Type
- Journal article