Journal article
A gene encoding a membrane protein exists upstream of the amoA/amoB genes in ammonia oxidizing bacteria: a third member of the amo operon?
FEMS microbiology letters, Vol.150(1), pp.65-73
1997
Handle:
https://hdl.handle.net/2376/112007
PMID: 9163908
Abstract
The gene cluster encoding ammonia monooxygenase (AMO) in the chemolithotrophic soil bacterium
Nitrosospira sp. NpAV was found to contain a third open reading frame, termed
amoC, upstream of the genes
amoA and
amoB that encode the subunits of AMO. The
amoC gene and its flanking regions were isolated and sequenced from a 4.4 kb
EcoRI fragment that contains one of three copies of the ammonia monooxygenase gene cluster. The presence of this gene upstream of the other two
amoA gene copies in
Nitrosospira NpAV as well as upstream of
amoA genes in the genomes of other ammonia oxidizing nitrifiers (strains in the genera
Nitrosomonas,
Nitrosospira,
Nitrosolobus and
Nitrosovibrio) was confirmed using genomic DNA, oligodeoxyribonucleotide primers and the PCR. The
amoC gene in
Nitrosospira sp. NpAV encodes a 270 amino acid polypeptide of approximately 36 kDa. Topological analysis of the predicted primary structure revealed 6 membrane spanning domains. The
amoC gene was expressed in recombinant
Escherichia coli from its indigenous promoter.
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Details
- Title
- A gene encoding a membrane protein exists upstream of the amoA/amoB genes in ammonia oxidizing bacteria: a third member of the amo operon?
- Creators
- Martin G Klotz - Department of Biology, Campus Box 171, University of Colorado, Denver, CO 80217-3364, USAJavier Alzerreca - Department of Plants, Soils and Biometeorology, Utah State University, Logan, UT 84322-4820, USAJeanette M Norton - Department of Plants, Soils and Biometeorology, Utah State University, Logan, UT 84322-4820, USA
- Publication Details
- FEMS microbiology letters, Vol.150(1), pp.65-73
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547702401842
- Language
- English
- Resource Type
- Journal article