Journal article
A genetic linkage map of an apple rootstock progeny anchored to the Malus genome sequence
Tree genetics & genomes, Vol.8(5), pp.991-1002
10/2012
Handle:
https://hdl.handle.net/2376/105899
Abstract
An apple rootstock progeny raised from the cross between the very dwarfing ‘M.27’ and the more vigorous ‘M.116’ (‘M.M.106’ × ‘M.27’) was used for the construction of a linkage map comprising a total of 324 loci: 252 previously mapped SSRs, 71 newly characterised or previously unmapped SSR loci (including 36 amplified by 33 out of the 35 novel markers reported here), and the self-incompatibility locus. The map spanned the 17 linkage groups (LG) expected for apple covering a genetic distance of 1,229.5 cM, an estimated 91% of the Malus genome. Linkage groups were well populated and, although marker density ranged from 2.3 to 6.2 cM/SSR, just 15 gaps of more than 15 cM were observed. Moreover, only 17.5% of markers displayed segregation distortion and, unsurprisingly in a semi-compatible backcross, distortion was particularly pronounced surrounding the self-incompatibility locus (S) at the bottom of LG17. DNA sequences of 273 SSR markers and the S locus, representing a total of 314 loci in this investigation, were used to anchor to the ‘Golden Delicious’ genome sequence. More than 260 of these loci were located on the expected pseudo-chromosome on the ‘Golden Delicious’ genome or on its homeologous pseudo-chromosome. In total, 282.4 Mbp of sequence from 142 genome sequence scaffolds of the Malus genome were anchored to the ‘M.27’ × ‘M.116’ map, providing an interface between the marker data and the underlying genome sequence. This will be exploited for the identification of genes responsible for traits of agronomic importance such as dwarfing and water use efficiency.
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Details
- Title
- A genetic linkage map of an apple rootstock progeny anchored to the Malus genome sequence
- Creators
- F Fernández-Fernández - East Malling Research (EMR) New Road, East Malling Kent ME19 6BJ UKL Antanaviciute - East Malling Research (EMR) New Road, East Malling Kent ME19 6BJ UKM van Dyk - Department of Genetics, Forestry and Agricultural Biotechnology Institute University of Pretoria Pretoria 0002 South AfricaK Tobutt - ARC Infruitec-Nietvoorbij Private Bag X5026 Stellenbosch 7599 South AfricaK Evans - WSU Tree Fruit Research & Extension Center 1100 N. Western Ave. Wenatchee WA 98801-1230 USAD Rees - ARC: Biotechnology Platform Private Bag X5, Onderstepoort Pretoria South AfricaJ Dunwell - School of Biological Sciences University of Reading Reading RG6 6AH UK1D Sargent - East Malling Research (EMR) New Road, East Malling Kent ME19 6BJ UK
- Publication Details
- Tree genetics & genomes, Vol.8(5), pp.991-1002
- Academic Unit
- WSU Wenatchee Tree Fruit REC
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900546606101842
- Language
- English
- Resource Type
- Journal article