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CC BY V4.0, Open Access
Journal article
A genome-wide association study of resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a worldwide collection of hexaploid spring wheat (Triticum aestivum L.)
G3 (Bethesda, Md.), Vol.5(3), pp.449-465
01/20/2015
Handle:
https://hdl.handle.net/2376/116422
PMCID: PMC4349098
PMID: 25609748
Abstract
New races of Puccinia striiformis f. sp. tritici (Pst), the causal pathogen of wheat stripe rust, show high virulence to previously deployed resistance genes and are responsible for large yield losses worldwide. To identify new sources of resistance we performed a genome-wide association study (GWAS) using a worldwide collection of 1000 spring wheat accessions. Adult plants were evaluated under field conditions in six environments in the western United States, and seedlings were tested with four Pst races. A single-nucleotide polymorphism (SNP) Infinium 9K-assay provided 4585 SNPs suitable for GWAS. High correlations among environments and high heritabilities were observed for stripe rust infection type and severity. Greater levels of Pst resistance were observed in a subpopulation from Southern Asia than in other groups. GWAS identified 97 loci that were significant for at least three environments, including 10 with an experiment-wise adjusted Bonferroni probability < 0.10. These 10 quantitative trait loci (QTL) explained 15% of the phenotypic variation in infection type, a percentage that increased to 45% when all QTL were considered. Three of these 10 QTL were mapped far from previously identified Pst resistance genes and QTL, and likely represent new resistance loci. The other seven QTL mapped close to known resistance genes and allelism tests will be required to test their relationships. In summary, this study provides an integrated view of stripe rust resistance resources in spring wheat and identifies new resistance loci that will be useful to diversify the current set of resistance genes deployed to control this devastating disease.
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- Title
- A genome-wide association study of resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a worldwide collection of hexaploid spring wheat (Triticum aestivum L.)
- Creators
- Marco Maccaferri - Department of Plant Sciences, University of California, Davis, California 95616 Department of Agricultural Sciences (DipSA), University of Bologna, Bologna 40127, ItalyJunli Zhang - Department of Plant Sciences, University of California, Davis, California 95616Peter Bulli - Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164-6420Zewdie Abate - Department of Plant Sciences, University of California, Davis, California 95616Shiaoman Chao - USDA-ARS, 1605 Albrecht Blvd, Fargo, North Dakota 58105Dario Cantu - Department of Viticulture and Enology, University of California, Davis, California 95616Eligio Bossolini - Department of Plant Sciences, University of California, Davis, California 95616Xianming Chen - USDA-ARS, Wheat Genetics, Quality Physiology, and Disease Research Unit, and Department of Plant Pathology, Washington State University, Pullman, Washington 99164Michael Pumphrey - Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164-6420Jorge Dubcovsky - Department of Plant Sciences, University of California, Davis, California 95616 Howard Hughes Medical Institute, Chevy Chase, Maryland 20815 Jdubcovsky@ucdavis.edu
- Publication Details
- G3 (Bethesda, Md.), Vol.5(3), pp.449-465
- Academic Unit
- Department of Plant Pathology ; Department of Crop and Soil Sciences
- Publisher
- United States
- Grant note
- Howard Hughes Medical Institute
- Identifiers
- 99900547877801842
- Language
- English
- Resource Type
- Journal article