A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice

Patricia M Manosalva; Rebecca M Davidson; Bin Liu; Xiaoyuan Zhu; Scot H Hulbert; Hei Leung; Jan E Leach

doi:10.1104/pp.108.128348

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A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice

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A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice

Patricia M Manosalva, Rebecca M Davidson, Bin Liu, Xiaoyuan Zhu, Scot H Hulbert, Hei Leung and Jan E Leach

Plant physiology (Bethesda), Vol.149(1), pp.286-296

01/2009

DOI: https://doi.org/10.1104/pp.108.128348

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https://hdl.handle.net/2376/105984

PMID: 19011003

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Abstract

Glycoproteins - genetics

Promoter Regions, Genetic

Multigene Family

Plants, Genetically Modified - genetics

Genes, Plant

Oryza - metabolism

Glycoproteins - metabolism

Gene Expression Profiling

Phylogeny

Immunity, Innate

Plant Proteins - genetics

Plants, Genetically Modified - metabolism

Plants, Genetically Modified - microbiology

Oryza - genetics

RNA Interference

Oryza - microbiology

Plant Diseases - genetics

Plant Proteins - metabolism

Quantitative Trait Loci

DNA, Plant - genetics

Plant disease resistance governed by quantitative trait loci (QTL) is predicted to be effective against a broad spectrum of pathogens and long lasting. Use of these QTL to improve crop species, however, is hindered because the genes contributing to the trait are not known. Five disease resistance QTL that colocalized with defense response genes were accumulated by marker-aided selection to develop blast-resistant varieties. One advanced backcross line carrying the major-effect QTL on chromosome (chr) 8, which included a cluster of 12 germin-like protein (OsGLP) gene members, exhibited resistance to rice (Oryza sativa) blast disease over 14 cropping seasons. To determine if OsGLP members contribute to resistance and if the resistance was broad spectrum, a highly conserved portion of the OsGLP coding region was used as an RNA interference trigger to silence a few to all expressed chr 8 OsGLP family members. Challenge with two different fungal pathogens (causal agents of rice blast and sheath blight diseases) revealed that as more chr 8 OsGLP genes were suppressed, disease susceptibility of the plants increased. Of the 12 chr 8 OsGLPs, one clustered subfamily (OsGER4) contributed most to resistance. The similarities of sequence, gene organization, and roles in disease resistance of GLP family members in rice and other cereals, including barley (Hordeum vulgare) and wheat (Triticum aestivum), suggest that resistance contributed by the chr 8 OsGLP is a broad-spectrum, basal mechanism conserved among the Gramineae. Natural selection may have preserved a whole gene family to provide a stepwise, flexible defense response to pathogen invasion.

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Title: A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice
Creators: Patricia M Manosalva - Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado 80523-1177, USA
Rebecca M Davidson
Bin Liu
Xiaoyuan Zhu
Scot H Hulbert
Hei Leung
Jan E Leach
Publication Details: Plant physiology (Bethesda), Vol.149(1), pp.286-296
Academic Unit: Department of Plant Pathology
Publisher: Amer Soc Plant Biologists; United States
Number of pages: 11
Grant note: Cereal Comparative Genomics Initiative International Rice Research Institute; CGIAR Kansas State and Colorado State Experiment Stations Guangdong Academy of Agricultural Sciences Ford Foundation Diversity Fellowship 2003-01551; 2004-35317-14867 / U.S. Department of Agriculture-Cooperative State Research, Extension, and Education Service-National Research Initiative; United States Department of Agriculture (USDA)
Identifiers: 99900546993701842
Language: English
Resource Type: Journal article