Journal article
A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)
BioSystems, Vol.35(2), pp.183-188
1995
Handle:
https://hdl.handle.net/2376/104176
PMID: 7488713
Abstract
Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.
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Details
- Title
- A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)
- Creators
- Mete Eray - School of Electrical Engineering and Computer Science, Washington State University, Pullman, WA 99164-2752, USANuman S Dogan - School of Electrical Engineering and Computer Science, Washington State University, Pullman, WA 99164-2752, USASteven R Reiken - Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USAHimawan Sutisna - Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USABernard J Van Wie - Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USAAlan R Koch - Department of Zoology, Washington State University, Pullman, WA 99164-4236, USADavid F Moffett - Department of Zoology, Washington State University, Pullman, WA 99164-4236, USAMoris Silber - Department of Zoology, Washington State University, Pullman, WA 99164-4236, USAWilliam C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA
- Publication Details
- BioSystems, Vol.35(2), pp.183-188
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier Ireland Ltd
- Identifiers
- 99900546848601842
- Language
- English
- Resource Type
- Journal article