Journal article
A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation
Process biochemistry (1991), Vol.42(11), pp.1537-1545
2007
Handle:
https://hdl.handle.net/2376/111112
Abstract
Crude glycerol is the primary by-product in the biodiesel industry, which is too costly to be purified into to higher quality products used in the health and cosmetics industries. This work investigated the potential of using the crude glycerol to produce docosahexaenoic acid (DHA, 22:6
n-3) through fermentation of the microalga
Schizochytrium limacinum. The results showed that crude glycerol supported alga growth and DHA production, with 75–100
g/L concentration being the optimal range. Among other medium and environmental factors influencing DHA production, temperature, trace metal (PI) solution concentration, ammonium acetate, and NH
4Cl had significant effects (
P
<
0.1). Their optimal values were determined 30
mL/L of PI, 0.04
g/L of NH
4Cl, 1.0
g/L of ammonium acetate, and 19.2
°C. A highest DHA yield of 4.91
g/L with 22.1
g/L cell dry weight was obtained. The results suggested that biodiesel-derived crude glycerol is a promising feedstock for production of DHA from heterotrophic algal culture.
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Details
- Title
- A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation
- Creators
- Zhanyou Chi - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99163, United StatesDenver Pyle - Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, United StatesZhiyou Wen - Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, United StatesCraig Frear - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99163, United StatesShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99163, United States
- Publication Details
- Process biochemistry (1991), Vol.42(11), pp.1537-1545
- Publisher
- Elsevier Ltd
- Identifiers
- 99900582324301842
- Language
- English
- Resource Type
- Journal article