Journal article
A magnetic electrochemical immunosensor for the detection of phosphorylated p53 based on enzyme functionalized carbon nanospheres with signal amplification
RSC advances, Vol.4(96), pp.54066-54071
01/01/2014
Abstract
Protein phosphorylation plays an important role in many biological processes and might be used as a potential biomarker in clinical diagnoses. We reported the development of a nanomaterial enhanced disposable immunosensor for ultrasensitive detection of phosphorylated p53 at Ser392 (phospho-p53(392)) using enzyme functionalization of carbon nanospheres (CNSs) as a signal amplification label and magnetic beads (MB) coupled with screen printed carbon electrodes as electrochemical transducers. In this work, horseradish peroxidase (HRP) and phospho-p53(392) detection antibody (Ab(2)) were co-linked to CNSs (HRP-CNSs-Ab(2)) for signal amplification, and functionalized MB was used as a platform to capture a large amount of primary antibodies (Ab(1)). The proposed signal amplification strategy with a sandwich-type immunoreaction significantly enhanced the sensitivity of detection of biomarkers. Under optimal conditions, the immunosensor had a highly linear voltammetric response to the phospho-p53(392) concentration in the range of 0.01 to 5 ng mL(-1), with a detection limit of 3.3 pg mL(-1). The results provided great potential for point-of-care detection of other phosphorylated proteins and clinical applications.
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Details
- Title
- A magnetic electrochemical immunosensor for the detection of phosphorylated p53 based on enzyme functionalized carbon nanospheres with signal amplification
- Creators
- Yanan Luo - Central China Normal UniversityAbdullah Mohamed Asiri - King Abdulaziz UniversityXiao Zhang - Central China Normal UniversityGuohai Yang - Washington State UniversityDan Du - Washington State UniversityYuehe Lin - King Abdulaziz University
- Publication Details
- RSC advances, Vol.4(96), pp.54066-54071
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Royal Soc Chemistry
- Number of pages
- 6
- Grant note
- 21275062 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) NCET-12-0871 / Program for New Century Excellent Talents in University; Program for New Century Excellent Talents in University (NCET) WSU start-up grant
- Identifiers
- 99901227847001842
- Language
- English
- Resource Type
- Journal article